2D/3D cryo x-ray fluorescence imaging at the bionanoprobe at the advanced photon source

Journal Article · Thu Jan 28 00:00:00 EST 2016 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4937522· OSTI ID:22494393

Vine, D. J.; Lai, B. ^[1]; Paunesku, T.; Yuan, Y.; Woloschak, G. E. ^[2]; Deng, J. ^[3]; Jin, Q.; Hong, Y. P. ^[4]; Flachenecker, C.; Hornberger, B. ^[5]; Brister, K. ^[6]; Jacobsen, C. ^[1]; Vogt, S. ^[1]

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1. Advanced Photon Source, Argonne National Laboratory, Lemont, IL 60439 (United States)
2. Department of Radiation Oncology, Northwester University, Chicago, IL 60611 (United States)
3. Applied Physics, Northwestern University, Evanston, IL 60208 (United States)
4. Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208 (United States)
5. Carl Zeiss X-ray Microscopy, Pleasanton, CA 94588 (United States)
6. Synchrotron Research Center, Northwestern University, Argonne, IL 60439 (United States)

Trace elements, particularly metals, play very important roles in biological systems. Synchrotron-based hard X-ray fluorescence microscopy offers the most suitable capabilities to quantitatively study trace metals in thick biological samples, such as whole cells and tissues. In this manuscript, we have demonstrated X-ray fluorescence imaging of frozen-hydrated whole cells using the recent developed Bionanoprobe (BNP). The BNP provides spatial resolution down to 30 nm and cryogenic capabilities. Frozen-hydrated biological cells have been directly examined on a sub-cellular level at liquid nitrogen temperatures with minimal sample preparation.

• OSTI ID: 22494393
• Journal Information: AIP Conference Proceedings, Vol. 1696, Issue 1; Conference: XRM 2014: 12. international conference on X-ray microscopy, Melbourne (Australia), 26-31 Oct 2014; Other Information: (c) 2016 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
ADVANCED PHOTON SOURCE
ANIMAL TISSUES
FLUORESCENCE
HARD X RADIATION
IMAGES
LIQUEFIED GASES
METALS
MICROSCOPY
NITROGEN
SAMPLE PREPARATION
SPATIAL RESOLUTION
SYNCHROTRONS
TRACE AMOUNTS