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Title: Three-dimensional structure of brain tissue at submicrometer resolution

Biological objects are composed of submicrometer structures such as cells and organelles that are essential for their functions. Here, we report on three-dimensional X-ray visualization of cells and organelles at resolutions up to 100 nm by imaging microtomography (micro-CT) equipped with Fresnel zone plate optics. Human cerebral tissue, fruit fly cephalic ganglia, and Escherichia coli bacteria labeled with high atomic-number elements were embedded in epoxy resin and subjected to X-ray microtomography at the BL37XU and BL47XU beamlines of the SPring-8 synchrotron radiation facility. The obtained results indicated that soft tissue structures can be visualized with the imaging microtomography.
Authors:
;  [1] ; ; ; ; ;  [2] ; ; ;  [3] ; ; ; ;  [4]
  1. Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa 259-1292 (Japan)
  2. Tokai University School of Medicine, Isehara, Kanagawa 259-1193 (Japan)
  3. Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506 (Japan)
  4. Japan Synchrotron Radiation Research Institute (JASRI/SPring-8), Sayo, Hyogo 679-5198 (Japan)
Publication Date:
OSTI Identifier:
22494375
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1696; Journal Issue: 1; Conference: XRM 2014: 12. international conference on X-ray microscopy, Melbourne (Australia), 26-31 Oct 2014; Other Information: (c) 2016 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; ANIMAL TISSUES; BIOMEDICAL RADIOGRAPHY; BRAIN; CELL CONSTITUENTS; COMPUTERIZED TOMOGRAPHY; EPOXIDES; ESCHERICHIA COLI; HUMAN POPULATIONS; RESINS; RESOLUTION; SPRING-8 STORAGE RING; SYNCHROTRON RADIATION; THREE-DIMENSIONAL CALCULATIONS; X RADIATION