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Title: Optimization of protein cross-linking in bicomponent electrospun scaffolds for therapeutic use

Bio-instructive electrospun scaffolds based on the combination of synthetic polymers, such as PCL or PLLA, and natural polymers (e.g., collagen) have been extensively investigated as temporary extracellular matrix (ECM) analogues able to support cell proliferation and stem cell differentiation for the regeneration of several tissues. The growing use of natural polymers as carrier of bioactive molecules is introducing new ideas for the design of polymeric drug delivery systems based on electrospun fibers with improved bioavailability, therapeutic efficacy and programmed drug release. In particular, the release mechanism is driven by the use of water soluble proteins (i.e., collagen, gelatin) which fully degrade in in vitro microenvironment, thus delivering the active principles. However, these protein are generally rapidly digested by enzymes (i.e., collagenase) produced by many different cell types, both in vivo and in vitro with significant drawbacks in tissue engineering and controlled drug delivery. Here, we aim at investigating different chemical strategies to improve the in vitro stability and mechanical strength of scaffolds against enzymatic degradation, by modifying the biodegradation rates of proteins embedded in bicomponent fibers. By comparing scaffolds treated by different cross-linking agents (i.e., GC, EDC, BDDGE), we have provided an extensive morphological/chemical/physical characterization via SEM and TGA tomore » identify the best conditions to control drug release via protein degradation from bicomponent fibers without compromising in vitro cell response.« less
Authors:
 [1] ;  [2] ; ; ; ;  [1]
  1. Institute for Polymers, Composites and Biomaterials, National Research Council of Italy (IPCB-CNR), V.le Kennedy 54, Naples 80125 (Italy)
  2. (Italy)
Publication Date:
OSTI Identifier:
22494359
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1695; Journal Issue: 1; Conference: GT70 international conference on polymer processing with resulting morphology and properties: Feet in the present and eyes at the future, Salerno (Italy), 15-17 Oct 2015; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANIMAL TISSUES; BIODEGRADATION; CELL PROLIFERATION; COLLAGEN; COMPARATIVE EVALUATIONS; CROSS-LINKING; DRUGS; ENZYMES; FIBERS; GELATIN; IN VITRO; IN VIVO; OPTIMIZATION; POLYMERS; SCANNING ELECTRON MICROSCOPY; STEM CELLS; THERAPEUTIC USES; THERMAL GRAVIMETRIC ANALYSIS