In vivo and in vitro toxicological effects of titanium dioxide nanoparticles on small intestine

Tassinari, Roberta; La Rocca, Cinzia; Tait, Sabrina; De Berardis, Barbara; Ammendolia, Maria Grazia; Iosi, Francesca; Di Virgilio, Antonio; Martinelli, Andrea; Maranghi, Francesca; Stecca, Laura

doi:10.1063/1.4922572

Title: In vivo and in vitro toxicological effects of titanium dioxide nanoparticles on small intestine

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Abstract

In European Union, titanium dioxide (TiO{sub 2}) as bulk material is a food additive (E171) and - as nanoparticle (NP) - is used as a white pigment in several products (e.g. food, cosmetics, drugs). E171 contains approximately 36% of particles less than 100 nm in at least one dimension and TiO{sub 2} NP exposure is estimated fairly below 2.5 mg/person/day. The gastrointestinal tract is a route of entry for NPs, thus representing a potential target of effects. In in vivo study, the effects of TiO{sub 2} NP in adult rat small intestine have been evaluated by oral administration of 0 (CTRL), 1 and 2 mg/kg body weight per day - relevant to human dietary intake. Detailed quali/quantitative histopathological analyses were performed on CTRL and treated rat samples. Scanning electron microscopy (SEM) analysis was performed on small intestine. An in vitro study on Caco-2 cells was also used in order to evaluate the potential cytotoxic effects directly on enterocytes through the lactate dehydrogenase (LDH) assay. Suspensions of TiO{sub 2} NPs for in vitro and in vivo study were characterized by EM. Histomorphometrical data showed treatment-related changes of villus height and widths in male rats. Significantly different from CTRL decreased LDH levels in the mediummore »« less

Authors:

Tassinari, Roberta; La Rocca, Cinzia; Tait, Sabrina; De Berardis, Barbara; Ammendolia, Maria Grazia; Iosi, Francesca; Di Virgilio, Antonio; Martinelli, Andrea; Maranghi, Francesca ^[1]; Stecca, Laura ^[2]

Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome (Italy)
European Commission-Joint Research Centre, Institute for Health and Consumer Protection Chemical assessment and Testing Unit, Via E. Fermi, 2749I-21027 Ispra (Italy)

Publication Date:: Tue Jun 23 00:00:00 EDT 2015

OSTI Identifier:: 22490686

Resource Type:: Journal Article

Journal Name:: AIP Conference Proceedings

Additional Journal Information:: Journal Volume: 1667; Journal Issue: 1; Conference: Nanoforum 2014, Rome (Italy), 22-25 Sep 2014; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0094-243X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY; ADULTS; CONSUMER PRODUCTS; EUROPEAN UNION; FOOD; FOOD ADDITIVES; HUMAN POPULATIONS; IN VITRO; IN VIVO; LACTATE DEHYDROGENASE; NANOPARTICLES; ORAL ADMINISTRATION; PIGMENTS; RATS; SCANNING ELECTRON MICROSCOPY; SMALL INTESTINE; SUSPENSIONS; TITANIUM OXIDES; TOXICITY

Citation Formats


                    Tassinari, Roberta, La Rocca, Cinzia, Tait, Sabrina, De Berardis, Barbara, Ammendolia, Maria Grazia, Iosi, Francesca, Di Virgilio, Antonio, Martinelli, Andrea, Maranghi, Francesca, and Stecca, Laura. In vivo and in vitro toxicological effects of titanium dioxide nanoparticles on small intestine.  United States: N. p., 2015. 
        Web.  doi:10.1063/1.4922572.

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                    Tassinari, Roberta, La Rocca, Cinzia, Tait, Sabrina, De Berardis, Barbara, Ammendolia, Maria Grazia, Iosi, Francesca, Di Virgilio, Antonio, Martinelli, Andrea, Maranghi, Francesca, & Stecca, Laura. In vivo and in vitro toxicological effects of titanium dioxide nanoparticles on small intestine.  United States.  https://doi.org/10.1063/1.4922572

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                    Tassinari, Roberta, La Rocca, Cinzia, Tait, Sabrina, De Berardis, Barbara, Ammendolia, Maria Grazia, Iosi, Francesca, Di Virgilio, Antonio, Martinelli, Andrea, Maranghi, Francesca, and Stecca, Laura. 2015.  
        "In vivo and in vitro toxicological effects of titanium dioxide nanoparticles on small intestine".  United States.  https://doi.org/10.1063/1.4922572.

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@article{osti_22490686,

  title        = {In vivo and in vitro toxicological effects of titanium dioxide nanoparticles on small intestine},

  author       = {Tassinari, Roberta and La Rocca, Cinzia and Tait, Sabrina and De Berardis, Barbara and Ammendolia, Maria Grazia and Iosi, Francesca and Di Virgilio, Antonio and Martinelli, Andrea and Maranghi, Francesca and Stecca, Laura},

  abstractNote = {In European Union, titanium dioxide (TiO{sub 2}) as bulk material is a food additive (E171) and - as nanoparticle (NP) - is used as a white pigment in several products (e.g. food, cosmetics, drugs). E171 contains approximately 36% of particles less than 100 nm in at least one dimension and TiO{sub 2} NP exposure is estimated fairly below 2.5 mg/person/day. The gastrointestinal tract is a route of entry for NPs, thus representing a potential target of effects. In in vivo study, the effects of TiO{sub 2} NP in adult rat small intestine have been evaluated by oral administration of 0 (CTRL), 1 and 2 mg/kg body weight per day - relevant to human dietary intake. Detailed quali/quantitative histopathological analyses were performed on CTRL and treated rat samples. Scanning electron microscopy (SEM) analysis was performed on small intestine. An in vitro study on Caco-2 cells was also used in order to evaluate the potential cytotoxic effects directly on enterocytes through the lactate dehydrogenase (LDH) assay. Suspensions of TiO{sub 2} NPs for in vitro and in vivo study were characterized by EM. Histomorphometrical data showed treatment-related changes of villus height and widths in male rats. Significantly different from CTRL decreased LDH levels in the medium were detected in vitro at 24h with 2.5, 5, 10 and 20 µg/cm{sup 2} levels of TiO{sub 2} NPs. SEM analysis showed no damaged areas. Overall the results showed that enterocytes may represent a target of TiO{sub 2} NP toxicity by direct exposure both in vivo and in vitro models.},

  doi          = {10.1063/1.4922572},

  url          = {https://www.osti.gov/biblio/22490686},
  journal      = {AIP Conference Proceedings},

  issn         = {0094-243X},

  number       = 1,

  volume       = 1667,

  place        = {United States},

  year         = {Tue Jun 23 00:00:00 EDT 2015},

  month        = {Tue Jun 23 00:00:00 EDT 2015}

}

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