Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes: A low frequency ESR study

Journal Article · Wed Jun 24 00:00:00 EDT 2015 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4917641· OSTI ID:22490217

Meenakumari, V.; Benial, A. Milton Franklin, ^[1]; Utsumi, Hideo; Ichikawa, Kazuhiro; Yamada, Ken-ichi ^[2]; Hyodo, Fuminori ^[3]; Jawahar, A. ^[4]

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1. Department of Physics, NMSSVN College, Nagamalai, Madurai-625019, Tamilnadu (India)
2. Department of Bio-functional Science, Kyushu University, Fukuoka (Japan)
3. Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka (Japan)
4. Department of Chemistry, NMSSVN College, Nagamalai, Madurai-625019, Tamilnadu (India)

Electron spin resonance (ESR) studies were carried out for permeable 2mM {sup 14}N-labeled deutrated 3 Methoxy carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 1mM, 2mM, 3mM, 4mM concentration of 14N-labeled deutrated MC-PROXYL in 400mM concentration of liposomal solution by using a 300 MHz ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported for these samples. The line broadening was observed for the nitroxyl spin probe in the liposomal solution. The line broadening indicates that the high viscous nature of the liposomal solution. The partition parameter and permeability values indicate the maximum diffusion of nitroxyl spin probes in the bilayer lipid membranes at 2 mM concentration of nitroxyl radical. This study illustrates that ESR can be used to differentiate between the intra and extra- membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the spin probe concentration was optimized as 2mM in liposomal solution for ESR phantom studies/imaging, invivo and invitro experiments.

• OSTI ID: 22490217
• Journal Information: AIP Conference Proceedings, Vol. 1665, Issue 1; Conference: 59. DAE solid state physics symposium 2014, Tamilnadu (India), 16-20 Dec 2014; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CARBONYLS
CONCENTRATION RATIO
COUPLING CONSTANTS
DIFFUSION
ELECTRON SPIN RESONANCE
LANDE FACTOR
LAYERS
LINE BROADENING
LINE WIDTHS
LIPIDS
MEMBRANES
MHZ RANGE
NITROGEN 14
NITROXYL RADICALS
PERMEABILITY
PYRROLIDINES