Intracellular effects of atmospheric-pressure plasmas on melanoma cancer cells

Bazaka, K.; Ostrikov, K.

doi:10.1063/1.4933366

Title: Intracellular effects of atmospheric-pressure plasmas on melanoma cancer cells

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Abstract

Gas discharge plasmas formed at atmospheric pressure and near room temperature have recently been shown as a promising tool for cancer treatment. The mechanism of the plasma action is attributed to generation of reactive oxygen and nitrogen species, electric fields, charges, and photons. The relative importance of different modes of action of atmospheric-pressure plasmas depends on the process parameters and specific treatment objects. Hence, an in-depth understanding of biological mechanisms that underpin plasma-induced death in cancer cells is required to optimise plasma processing conditions. Here, the intracellular factors involved in the observed anti-cancer activity in melanoma Mel007 cells are studied, focusing on the effect of the plasma treatment dose on the expression of tumour suppressor protein TP73. Over-expression of TP73 causes cell growth arrest and/or apoptosis, and hence can potentially be targeted to enhance killing efficacy and selectivity of the plasma treatment. It is shown that the plasma treatment induces dose-dependent up-regulation of TP73 gene expression, resulting in significantly elevated levels of TP73 RNA and protein in plasma-treated melanoma cells. Silencing of TP73 expression by means of RNA interference inhibited the anticancer effects of the plasma, similar to the effect of caspase inhibitor z-VAD or ROS scavenger N-acetyl cysteine. Thesemore »« less

Authors:

Bazaka, K. ^[1]; Ostrikov, K. ^[2]

Institute for Health and Biomedical Innovation, School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, QLD 4000 (Australia)
Comonwealth Scientific and Industrial Research Organization, Sydney, New South Wales (Australia)

Publication Date:: Tue Dec 15 00:00:00 EST 2015

OSTI Identifier:: 22489902

Resource Type:: Journal Article

Journal Name:: Physics of Plasmas

Additional Journal Information:: Journal Volume: 22; Journal Issue: 12; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1070-664X

Country of Publication:: United States

Language:: English

Subject:: 70 PLASMA PHYSICS AND FUSION TECHNOLOGY; APOPTOSIS; ATMOSPHERIC PRESSURE; CYSTEINE; DOSES; ELECTRIC FIELDS; INTERFERENCE; MELANOMAS; NITROGEN; OXYGEN; PHOTONS; PLASMA; RNA; TEMPERATURE RANGE 0273-0400 K

Citation Formats


                    Ishaq, M., E-mail: ishaqmusarat@gmail.com, Comonwealth Scientific and Industrial Research Organization, Sydney, New South Wales, Bazaka, K., Ostrikov, K., and Institute for Health and Biomedical Innovation, School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, QLD 4000. Intracellular effects of atmospheric-pressure plasmas on melanoma cancer cells.  United States: N. p., 2015. 
        Web.  doi:10.1063/1.4933366.

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                    Ishaq, M., E-mail: ishaqmusarat@gmail.com, Comonwealth Scientific and Industrial Research Organization, Sydney, New South Wales, Bazaka, K., Ostrikov, K., & Institute for Health and Biomedical Innovation, School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, QLD 4000. Intracellular effects of atmospheric-pressure plasmas on melanoma cancer cells.  United States.  https://doi.org/10.1063/1.4933366

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                    Ishaq, M., E-mail: ishaqmusarat@gmail.com, Comonwealth Scientific and Industrial Research Organization, Sydney, New South Wales, Bazaka, K., Ostrikov, K., and Institute for Health and Biomedical Innovation, School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, QLD 4000. 2015.  
        "Intracellular effects of atmospheric-pressure plasmas on melanoma cancer cells".  United States.  https://doi.org/10.1063/1.4933366.

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@article{osti_22489902,

  title        = {Intracellular effects of atmospheric-pressure plasmas on melanoma cancer cells},

  author       = {Ishaq, M., E-mail: ishaqmusarat@gmail.com and Comonwealth Scientific and Industrial Research Organization, Sydney, New South Wales and Bazaka, K. and Ostrikov, K. and Institute for Health and Biomedical Innovation, School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, QLD 4000},

  abstractNote = {Gas discharge plasmas formed at atmospheric pressure and near room temperature have recently been shown as a promising tool for cancer treatment. The mechanism of the plasma action is attributed to generation of reactive oxygen and nitrogen species, electric fields, charges, and photons. The relative importance of different modes of action of atmospheric-pressure plasmas depends on the process parameters and specific treatment objects. Hence, an in-depth understanding of biological mechanisms that underpin plasma-induced death in cancer cells is required to optimise plasma processing conditions. Here, the intracellular factors involved in the observed anti-cancer activity in melanoma Mel007 cells are studied, focusing on the effect of the plasma treatment dose on the expression of tumour suppressor protein TP73. Over-expression of TP73 causes cell growth arrest and/or apoptosis, and hence can potentially be targeted to enhance killing efficacy and selectivity of the plasma treatment. It is shown that the plasma treatment induces dose-dependent up-regulation of TP73 gene expression, resulting in significantly elevated levels of TP73 RNA and protein in plasma-treated melanoma cells. Silencing of TP73 expression by means of RNA interference inhibited the anticancer effects of the plasma, similar to the effect of caspase inhibitor z-VAD or ROS scavenger N-acetyl cysteine. These results confirm the role of TP73 protein in dose-dependent regulation of anticancer activity of atmospheric-pressure plasmas.},

  doi          = {10.1063/1.4933366},

  url          = {https://www.osti.gov/biblio/22489902},
  journal      = {Physics of Plasmas},

  issn         = {1070-664X},

  number       = 12,

  volume       = 22,

  place        = {United States},

  year         = {Tue Dec 15 00:00:00 EST 2015},

  month        = {Tue Dec 15 00:00:00 EST 2015}

}

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