skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy

Abstract

We present multi-frequency force modulation atomic force microscopy (AFM) for mapping the complex shear modulus G* of living cells as a function of frequency over the range of 50–500 Hz in the same measurement time as the single-frequency force modulation measurement. The AFM technique enables us to reconstruct image maps of rheological parameters, which exhibit a frequency-dependent power-law behavior with respect to G{sup *}. These quantitative rheological measurements reveal a large spatial variation in G* in this frequency range for single cells. Moreover, we find that the reconstructed images of the power-law rheological parameters are much different from those obtained in force-curve or single-frequency force modulation measurements. This indicates that the former provide information about intracellular mechanical structures of the cells that are usually not resolved with the conventional force measurement methods.

Authors:
;  [1]
  1. Graduate School of Information Science and Technology, Hokkaido University, Kita-ku N14 W9, Sapporo 060-0814 (Japan)
Publication Date:
OSTI Identifier:
22485978
Resource Type:
Journal Article
Journal Name:
Applied Physics Letters
Additional Journal Information:
Journal Volume: 107; Journal Issue: 17; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0003-6951
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; ATOMIC FORCE MICROSCOPY; DIAGRAMS; FREQUENCY DEPENDENCE; FREQUENCY RANGE; IMAGES; MAPPING; MAPS; MODULATION; RHEOLOGY; SHEAR

Citation Formats

Takahashi, Ryosuke, and Okajima, Takaharu. Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy. United States: N. p., 2015. Web. doi:10.1063/1.4934874.
Takahashi, Ryosuke, & Okajima, Takaharu. Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy. United States. https://doi.org/10.1063/1.4934874
Takahashi, Ryosuke, and Okajima, Takaharu. 2015. "Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy". United States. https://doi.org/10.1063/1.4934874.
@article{osti_22485978,
title = {Mapping power-law rheology of living cells using multi-frequency force modulation atomic force microscopy},
author = {Takahashi, Ryosuke and Okajima, Takaharu},
abstractNote = {We present multi-frequency force modulation atomic force microscopy (AFM) for mapping the complex shear modulus G* of living cells as a function of frequency over the range of 50–500 Hz in the same measurement time as the single-frequency force modulation measurement. The AFM technique enables us to reconstruct image maps of rheological parameters, which exhibit a frequency-dependent power-law behavior with respect to G{sup *}. These quantitative rheological measurements reveal a large spatial variation in G* in this frequency range for single cells. Moreover, we find that the reconstructed images of the power-law rheological parameters are much different from those obtained in force-curve or single-frequency force modulation measurements. This indicates that the former provide information about intracellular mechanical structures of the cells that are usually not resolved with the conventional force measurement methods.},
doi = {10.1063/1.4934874},
url = {https://www.osti.gov/biblio/22485978}, journal = {Applied Physics Letters},
issn = {0003-6951},
number = 17,
volume = 107,
place = {United States},
year = {Mon Oct 26 00:00:00 EDT 2015},
month = {Mon Oct 26 00:00:00 EDT 2015}
}