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Title: Significant accumulation of persistent organic pollutants and dysregulation in multiple DNA damage repair pathways in the electronic-waste-exposed populations

Electronic waste (e-waste) has created a worldwide environmental and health problem, by generating a diverse group of hazardous compounds such as persistent organic pollutants (POPs). Our previous studies demonstrated that populations from e-waste exposed region have a significantly higher level of chromosomal aberrancy and incidence of DNA damage. In this study, we further demonstrated that various POPs persisted at a significantly higher concentration in the exposed group than those in the unexposed group. The level of reactive oxygen species and micronucleus rate were also significantly elevated in the exposed group. RNA sequencing analysis revealed 31 genes in DNA damage responses and repair pathways that were differentially expressed between the two groups (Log 2 ratio >1 or <−1). Our data demonstrated that both females and males of the exposed group have activated a series of DNA damage response genes; however many important DNA repair pathways have been dysregulated. Expressions of NEIL1/3 and RPA3, which are critical in initiating base pair and nucleotide excision repairs respectively, have been downregulated in both females and males of the exposed group. In contrast, expression of RNF8, an E3 ligase involved in an error prone non-homologous end joining repair for DNA double strand break, was upregulatedmore » in both genders of the exposed group. The other genes appeared to be differentially expressed only when the males or females of the two groups were compared respectively. Importantly, the expression of cell cycle regulatory gene CDC25A that has been implicated in multiple kinds of malignant transformation was significantly upregulated among the exposed males while downregulated among the exposed females. In conclusion, our studies have demonstrated significant correlations between e-waste disposing and POPs accumulation, DNA lesions and dysregulation of multiple DNA damage repair mechanisms in the residents of the e-waste exposed region. - Highlights: • We compared concentration of POPs, ROS and micronucleus rate in POPs exposed area. • Significant accumulation of POPs homologous in the e-waste exposed residents. • DNA damage and DNA damage repair pathways have been differentially activated. • Females and males in the exposed group have different responses to the DNA damage. • Exposed males may be more prone to undergo malignant transformation.« less
Authors:
; ; ;  [1] ;  [2] ;  [1] ;  [3] ;  [4] ;  [5] ;  [3] ;  [1]
  1. Basic Medical College, Tianjin Medical University, Tianjin 300070 (China)
  2. Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Tianjin 300070 (China)
  3. State Key Joint Laboratory for Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering and Center for Environment and Health, Peking University, Beijing 100871 (China)
  4. Department of Breast Surgery, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060 (China)
  5. (China)
Publication Date:
OSTI Identifier:
22483284
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Research; Journal Volume: 137; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; ABUNDANCE; BIOLOGICAL PATHWAYS; BUILDUP; CELL CYCLE; COMPARATIVE EVALUATIONS; CONCENTRATION RATIO; CORRELATIONS; DNA; ECOLOGICAL CONCENTRATION; ERRORS; EXCISION REPAIR; FEMALES; GENES; JOINING; MALES; NUCLEOTIDES; OXYGEN; POLLUTANTS; POPULATIONS; RNA; STRAND BREAKS; TRANSFORMATIONS; WASTES