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Title: New scientific equipment for protein crystallization in microgravity, BELKA, and its approbation on the Bion-M No. 1 spacecraft

A space experiment on the crystallization of lisozyme and glucose isomerase proteins in UK-1 and UK-2 crystallizers on the scientific equipment BELKA on the Bion-M no. 1 spacecraft was performed in April–May 2013. A ground-based experiment was carried out simultaneously at the Institute of Crystallography of the Russian Academy of Sciences (IC RAS). Transparent crystals were obtained in both cases. The lisozyme crystals grown in microgravity are larger than their terrestrial analogs. An optical study of glucose isomerase crystals grown in space has shown that the coalescence of equally oriented crystallites leads to the formation of quasi-single-crystal blocks. An X-ray diffraction experiment on lisozyme crystals has revealed the resolutions for crystals obtained under terrestrial conditions and in space to be 1.74 and 1.58 Å, respectively.
Authors:
; ; ; ; ; ;  [1] ; ;  [2] ; ;  [1]
  1. Russian Academy of Sciences, Shubnikov Institute of Crystallography (Russian Federation)
  2. Center for Operation of Space Ground Based Infrastructure (Russian Federation)
Publication Date:
OSTI Identifier:
22472487
Resource Type:
Journal Article
Resource Relation:
Journal Name: Crystallography Reports; Journal Volume: 60; Journal Issue: 1; Other Information: Copyright (c) 2015 Pleiades Publishing, Inc.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; COALESCENCE; CRYSTAL GROWTH; CRYSTALLIZATION; GLUCOSE; ISOMERASES; MONOCRYSTALS; PROTEINS; SPACE VEHICLES; WEIGHTLESSNESS; X-RAY DIFFRACTION