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Title: Coronavirus envelope (E) protein remains at the site of assembly

Coronaviruses (CoVs) assemble at endoplasmic reticulum Golgi intermediate compartment (ERGIC) membranes and egress from cells in cargo vesicles. Only a few molecules of the envelope (E) protein are assembled into virions. The role of E in morphogenesis is not fully understood. The cellular localization and dynamics of mouse hepatitis CoV A59 (MHV) E protein were investigated to further understanding of its role during infection. E protein localized in the ERGIC and Golgi with the amino and carboxy termini in the lumen and cytoplasm, respectively. E protein does not traffic to the cell surface. MHV was genetically engineered with a tetracysteine tag at the carboxy end of E. Fluorescence recovery after photobleaching (FRAP) showed that E is mobile in ERGIC/Golgi membranes. Correlative light electron microscopy (CLEM) confirmed the presence of E in Golgi cisternae. The results provide strong support that E proteins carry out their function(s) at the site of budding/assembly. - Highlights: • Mouse hepatitis coronavirus (MHV-CoV) E protein localizes in the ERGIC and Golgi. • MHV-CoV E does not transport to the cell surface. • MHV-CoV can be genetically engineered with a tetracysteine tag appended to E. • First FRAP and correlative light electron microscopy of a CoV Emore » protein. • Live-cell imaging shows that E is mobile in ERGIC/Golgi membranes.« less
Authors:
 [1] ;  [2] ;  [2] ;  [1] ;  [2] ;  [1] ;  [2] ;  [2] ;  [1] ;  [2] ;  [2] ;  [1] ;  [2]
  1. The Biodesign Institute, Center for Infectious Diseases and Vaccinology, Arizona State University, Tempe, AZ 85287-5401 (United States)
  2. (United States)
Publication Date:
OSTI Identifier:
22470161
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 478; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL RECOVERY; COMPARTMENTS; CYTOPLASM; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; FLUORESCENCE; HEPATITIS; MEMBRANES; MICE; MOLECULES; MORPHOGENESIS; PROTEINS; SURFACES; VIRUSES