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Title: TYLCV-Is movement in planta does not require V2 protein

Tomato yellow leaf curl virus (TYLCV), a major tomato pathogen causing extensive crop losses, is a whitefly-transmitted geminivirus. V2 mutants of TYLCV-Is and related viruses tend to induce symptomless infection with attenuated viral DNA levels, while accumulating close to wild-type DNA levels in protoplasts, suggesting V2 as a movement protein. The discovery of plant-silencing mechanisms and viral silencing suppressors, V2 included, led us to reconsider V2's involvement in viral movement. We studied two mutant versions of the virus, one impaired in V2 silencing-suppression activity, and another carrying a non-translatable V2. While both mutant viruses spread in the infected plant to newly emerged leaves at the same rate as the wild-type virus, their DNA-accumulation levels were tenfold lower than in the wild-type virus. Thus, we suggest that the setback in virus proliferation, previously ascribed to a movement impediment, is due to lack of silencing-suppression activity. - Highlights: • TYLCV-Is V2 protein is localized in distinct microbodies throughout the cell cytoplasm, around the nucleus and in association with cytoplasmic strands but is not associated with the plasmodesmata. • Disruption of RNA-silencing suppression activity of TYLCV-Is V2 protein causes low titer of the virus in the infected plants. • The movement of TYLCV-Ismore » in planta does not require a functional V2 protein.« less
Authors:
 [1] ;  [2] ; ; ;  [1] ;  [3] ;  [1] ;  [1]
  1. Institute of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet Dagan (Israel)
  2. (Israel)
  3. Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem (Israel)
Publication Date:
OSTI Identifier:
22470157
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 477; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BUILDUP; CROPS; CYTOPLASM; DNA; INHIBITION; LEAVES; MUTANTS; PATHOGENS; PLANT CELLS; PLANTS; PROTEINS; RNA; TOMATOES; VIRUSES