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Title: Safety evaluation of intravenously administered mono-thioated aptamer against E-selectin in mice

Abstract

The medical applications of aptamers have recently emerged. We developed an antagonistic thioaptamer (ESTA) against E-selectin. Previously, we showed that a single injection of ESTA at a dose of 100 μg inhibits breast cancer metastasis in mice through the functional blockade of E-selectin. In the present study, we evaluated the safety of different doses of intravenously administered ESTA in single-dose acute and repeat-dose subacute studies in ICR mice. Our data indicated that intravenous administration of up to 500 μg ESTA did not result in hematologic abnormality in either study. Additionally, intravenous injection of ESTA did not affect the levels of plasma cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, GM-CSF, IFN-γ, and TNF-α) or complement split products (C3a and C5a) in either study. However, repeated injections of ESTA slightly increased plasma ALT and AST activities, in accordance with the appearance of small necrotic areas in the liver. In conclusion, our data demonstrated that intravenous administration of ESTA does not cause overt hematologic, organs, and immunologic responses under the experimental conditions. - Highlights: • Intravenous administration of ESTA was well tolerated. • ESTA up to 500 μg does not cause hematologic, organs, and immunologic responses. • ESTA-mediated hepatic abnormality was considered minor.

Authors:
;  [1];  [2]; ; ;  [1]; ;  [2]; ; ;  [1];  [3];  [4];  [2];  [1]
  1. Stephenson Cancer Center, University of Oklahoma Health Sciences Center, 975 NE, 10th, Oklahoma City, OK 73104 (United States)
  2. Institute of Molecular Medicine, Department of NanoMedicine and Biomedical Engineering, University of Texas Health Science Center at Houston, 1825 Hermann Pressler, Houston, TX 77030 (United States)
  3. Thomas Jefferson University, 1020 Locust St, Philadelphia, PA 19107 (United States)
  4. John Theurer Cancer Center, Hackensack University Medical Center, Hackensack, NJ 07601 (United States)
Publication Date:
OSTI Identifier:
22465808
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 287; Journal Issue: 1; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DOSES; INTRAVENOUS INJECTION; LIVER; LYMPHOKINES; MAMMARY GLANDS; METASTASES; MICE; NEOPLASMS; SAFETY ANALYSIS; TOXICITY

Citation Formats

Kang, Shin-Ae, Tsolmon, Bilegtsaikhan, Mann, Aman P., Zheng, Wei, Zhao, Lichao, Zhao, Yan Daniel, Volk, David E., Lokesh, Ganesh L.-R., Morris, Lynsie, Gupta, Vineet, Razaq, Wajeeha, Rui, Hallgeir, Suh, K. Stephen, Gorenstein, David G., and Tanaka, Takemi. Safety evaluation of intravenously administered mono-thioated aptamer against E-selectin in mice. United States: N. p., 2015. Web. doi:10.1016/J.TAAP.2015.05.011.
Kang, Shin-Ae, Tsolmon, Bilegtsaikhan, Mann, Aman P., Zheng, Wei, Zhao, Lichao, Zhao, Yan Daniel, Volk, David E., Lokesh, Ganesh L.-R., Morris, Lynsie, Gupta, Vineet, Razaq, Wajeeha, Rui, Hallgeir, Suh, K. Stephen, Gorenstein, David G., & Tanaka, Takemi. Safety evaluation of intravenously administered mono-thioated aptamer against E-selectin in mice. United States. https://doi.org/10.1016/J.TAAP.2015.05.011
Kang, Shin-Ae, Tsolmon, Bilegtsaikhan, Mann, Aman P., Zheng, Wei, Zhao, Lichao, Zhao, Yan Daniel, Volk, David E., Lokesh, Ganesh L.-R., Morris, Lynsie, Gupta, Vineet, Razaq, Wajeeha, Rui, Hallgeir, Suh, K. Stephen, Gorenstein, David G., and Tanaka, Takemi. 2015. "Safety evaluation of intravenously administered mono-thioated aptamer against E-selectin in mice". United States. https://doi.org/10.1016/J.TAAP.2015.05.011.
@article{osti_22465808,
title = {Safety evaluation of intravenously administered mono-thioated aptamer against E-selectin in mice},
author = {Kang, Shin-Ae and Tsolmon, Bilegtsaikhan and Mann, Aman P. and Zheng, Wei and Zhao, Lichao and Zhao, Yan Daniel and Volk, David E. and Lokesh, Ganesh L.-R. and Morris, Lynsie and Gupta, Vineet and Razaq, Wajeeha and Rui, Hallgeir and Suh, K. Stephen and Gorenstein, David G. and Tanaka, Takemi},
abstractNote = {The medical applications of aptamers have recently emerged. We developed an antagonistic thioaptamer (ESTA) against E-selectin. Previously, we showed that a single injection of ESTA at a dose of 100 μg inhibits breast cancer metastasis in mice through the functional blockade of E-selectin. In the present study, we evaluated the safety of different doses of intravenously administered ESTA in single-dose acute and repeat-dose subacute studies in ICR mice. Our data indicated that intravenous administration of up to 500 μg ESTA did not result in hematologic abnormality in either study. Additionally, intravenous injection of ESTA did not affect the levels of plasma cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, GM-CSF, IFN-γ, and TNF-α) or complement split products (C3a and C5a) in either study. However, repeated injections of ESTA slightly increased plasma ALT and AST activities, in accordance with the appearance of small necrotic areas in the liver. In conclusion, our data demonstrated that intravenous administration of ESTA does not cause overt hematologic, organs, and immunologic responses under the experimental conditions. - Highlights: • Intravenous administration of ESTA was well tolerated. • ESTA up to 500 μg does not cause hematologic, organs, and immunologic responses. • ESTA-mediated hepatic abnormality was considered minor.},
doi = {10.1016/J.TAAP.2015.05.011},
url = {https://www.osti.gov/biblio/22465808}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 1,
volume = 287,
place = {United States},
year = {Sat Aug 15 00:00:00 EDT 2015},
month = {Sat Aug 15 00:00:00 EDT 2015}
}