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Title: Changes in cholesterol homeostasis and acute phase response link pulmonary exposure to multi-walled carbon nanotubes to risk of cardiovascular disease

Adverse lung effects following pulmonary exposure to multi-walled carbon nanotubes (MWCNTs) are well documented in rodents. However, systemic effects are less understood. Epidemiological studies have shown increased cardiovascular disease risk after pulmonary exposure to airborne particles, which has led to concerns that inhalation exposure to MWCNTs might pose similar risks. We analyzed parameters related to cardiovascular disease, including plasma acute phase response (APR) proteins and plasma lipids, in female C57BL/6 mice exposed to a single intratracheal instillation of 0, 18, 54 or 162 μg/mouse of small, entangled (CNT{sub Small}, 0.8 ± 0.1 μm long) or large, thick MWCNTs (CNT{sub Large}, 4 ± 0.4 μm long). Liver tissues and plasma were harvested 1, 3 and 28 days post-exposure. In addition, global hepatic gene expression, hepatic cholesterol content and liver histology were used to assess hepatic effects. The two MWCNTs induced similar systemic responses despite their different physicochemical properties. APR proteins SAA3 and haptoglobin, plasma total cholesterol and low-density/very low-density lipoprotein were significantly increased following exposure to either MWCNTs. Plasma SAA3 levels correlated strongly with pulmonary Saa3 levels. Analysis of global gene expression revealed perturbation of the same biological processes and pathways in liver, including the HMG-CoA reductase pathway. Both MWCNTs inducedmore » similar histological hepatic changes, with a tendency towards greater response following CNT{sub Large} exposure. Overall, we show that pulmonary exposure to two different MWCNTs induces similar systemic and hepatic responses, including changes in plasma APR, lipid composition, hepatic gene expression and liver morphology. The results link pulmonary exposure to MWCNTs with risk of cardiovascular disease. - Highlights: • Systemic and hepatic alterations were evaluated in female mice following MWCNT instillation. • Despite being physicochemically different, the two MWCNTs induced a similar systemic acute phase response. • We found a close correlation between plasma SAA levels and pulmonary Saa3 transcription levels. • Similar changes in plasma lipid levels and in the hepatic transcriptome were observed. • Our results indicate a link between pulmonary exposure to MWCNTs with risk of cardiovascular disease.« less
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [4] ;  [5] ;  [5] ;  [6] ;  [1] ;  [5] ;  [1] ;  [2] ;  [5] ;  [1] ;  [2]
  1. National Research Centre for the Working Environment, DK-2100 Copenhagen (Denmark)
  2. (Denmark)
  3. National Food Institute, Technical University of Denmark, Søborg (Denmark)
  4. Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn (Poland)
  5. Environmental and Radiation Health Sciences Directorate, Health Canada, Ottawa, Ontario K1A 0K9 (Canada)
  6. Department of Science, Systems and Models, Roskilde University, DK-4000 Roskilde (Denmark)
Publication Date:
OSTI Identifier:
22465716
Resource Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 283; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; ARTERIOSCLEROSIS; CARBON NANOTUBES; CHOLESTEROL; DISTURBANCES; FEMALES; GENES; HISTOLOGY; HOMEOSTASIS; IMMUNITY; INHALATION; LIPOPROTEINS; LIVER; LUNGS; MICE; MORPHOLOGY; PARTICULATES; PERTURBATION THEORY; TRANSCRIPTION