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Title: MiR-20a Induces Cell Radioresistance by Activating the PTEN/PI3K/Akt Signaling Pathway in Hepatocellular Carcinoma

Purpose: To investigate the role of miR-20a in hepatocellular carcinoma (HCC) cell radioresistance, which may reveal potential strategies to improve treatment. Methods and Materials: The expression of miR-20a and PTEN were detected in HCC cell lines and paired primary tissues by quantitative real-time polymerase chain reaction. Cell radiation combined with colony formation assays was administrated to discover the effect of miR-20a on radiosensitivity. Bioinformatics prediction and luciferase assay were used to identify the target of miR-20a. The phosphatidylinositol 3-kinase inhibitor LY294002 was used to inhibit phosphorylation of Akt, to verify whether miR-20a affects HCC cell radioresistance through activating the PTEN/PI3K/Akt pathway. Results: MiR-20a levels were increased in HCC cell lines and tissues, whereas PTEN was inversely correlated with it. Overexpression of miR-20a in Bel-7402 and SMMC-7721 cells enhances their resistance to the effect of ionizing radiation, and the inhibition of miR-20a in HCCLM3 and QGY-7701 cells sensitizes them to it. PTEN was identified as a direct functional target of miR-20a for the induction of radioresistance. Overexpression of miR-20a activated the PTEN/PI3K/Akt signaling pathway. Additionally, the kinase inhibitor LY294002 could reverse the effect of miR-20a–induced radioresistance. Conclusion: MiR-20a induces HCC cell radioresistance by activating the PTEN/PI3K/Akt pathway, which suggests that miR-20a/PTEN/PI3K/Aktmore » might represent a target of investigation for developing effective therapeutic strategies against HCC.« less
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [1] ; ;  [5] ;  [6] ;  [1] ;  [1] ;  [1]
  1. Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province (China)
  2. Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province (China)
  3. (China)
  4. Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province (China)
  5. Department of Radiation Oncology, Cancer Hospital, Hangzhou, Zhejiang Province (China)
  6. Department of Radiation Oncology, Nanhai Hospital, Southern Medical University, Guangzhou, Guangdong Province (China)
Publication Date:
OSTI Identifier:
22462411
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Radiation Oncology, Biology and Physics; Journal Volume: 92; Journal Issue: 5; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; ADMINISTRATIVE PROCEDURES; ANIMAL TISSUES; COLONY FORMATION; HEPATOMAS; LUCIFERASE; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; RADIOSENSITIVITY; SIGNALS