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Title: Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro

The transcription factor Nuclear Factor I-C (NFIC) has been implicated in the regulation of tooth root development, where it may be anticipated to impact on the behavior of stem cells from the apical papilla (SCAPs) and root odontoblast activity. We hypothesized that NFIC may provide an important target for promoting dentin/root regeneration. In the present study, the effects of NFIC on the proliferation and differentiation of SCAPs were investigated. Over-expression of NFIC increased cell proliferation, mineralization nodule formation and alkaline phosphatase (ALP) activity in SCAPs. Furthermore, NFIC up-regulated the mRNA levels of odontogenic-related markers, ALP, osteocalcin and collagen type I as well as dentin sialoprotein protein levels. In contrast, knockdown of NFIC by si-RNA inhibited the mineralization capacity of SCAPs and down-regulated the expression of odontogenic-related markers. In conclusion, the results indicated that upregulation of NFIC activity in SCAPs may promote osteo/odontoblastic differentiation of SCAPs. - Highlights: • NFIC promotes the proliferation of SCAPs in vitro. • NFIC promotes osteo/odontogenic differentiation of SCAPs in vitro. • Knockdown of NFIC inhibits odontogenic differentiation in SCAPs.
Authors:
 [1] ;  [2] ; ;  [1] ;  [3] ; ;  [1] ; ;  [4] ;  [1]
  1. State Key Laboratory of Military Stomatology, Department of Operative Dentistry & Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an (China)
  2. (China)
  3. Treatment center of oral diseases, The 306th Hospital of People's Liberation Army, Beijing (China)
  4. Oral Biology, School of Dentistry, University of Birmingham, B4 6NN (United Kingdom)
Publication Date:
OSTI Identifier:
22462274
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 332; Journal Issue: 2; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALKALINE PHOSPHATASE; CELL PROLIFERATION; COLLAGEN; DENTIN; HUMAN POPULATIONS; IN VITRO; MESSENGER-RNA; MINERALIZATION; STEM CELLS; TEETH; TRANSCRIPTION FACTORS