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Title: Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis

Abstract

We previously demonstrated that the expression of intercellular adhesion molecule-1 (ICAM-1) by skeletal muscle cells after muscle overload contributes to ensuing regenerative and hypertrophic processes in skeletal muscle. The objective of the present study is to reveal mechanisms through which skeletal muscle cell expression of ICAM-1 augments regenerative and hypertrophic processes of myogenesis. This was accomplished by genetically engineering C2C12 myoblasts to stably express ICAM-1, and by inhibiting the adhesive and signaling functions of ICAM-1 through the use of a neutralizing antibody or cell penetrating peptide, respectively. Expression of ICAM-1 by cultured skeletal muscle cells augmented myoblast–myoblast adhesion, myotube formation, myonuclear number, myotube alignment, myotube–myotube fusion, and myotube size without influencing the ability of myoblasts to proliferate or differentiate. ICAM-1 augmented myotube formation, myonuclear accretion, and myotube alignment through a mechanism involving adhesion-induced activation of ICAM-1 signaling, as these dependent measures were reduced via antibody and peptide inhibition of ICAM-1. The adhesive and signaling functions of ICAM-1 also facilitated myotube hypertrophy through a mechanism involving myotube–myotube fusion, protein synthesis, and Akt/p70s6k signaling. Our findings demonstrate that ICAM-1 expression by skeletal muscle cells augments myogenesis, and establish a novel mechanism through which the inflammatory response facilitates growth processes in skeletal muscle.more » - Highlights: • We examined mechanisms through which skeletal muscle cell expression of ICAM-1 facilitates events of in vitro myogenesis. • Expression of ICAM-1 by cultured myoblasts did not influence their ability to proliferate or differentiate. • Skeletal muscle cell expression of ICAM-1 augmented myoblast fusion, myotube alignment, myotube–myotube fusion, and myotube size. • ICAM-1 augmented myogenic processes through mechanisms involving its adhesive and signaling functions.« less

Authors:
; ;  [1];  [2];  [3]
  1. Department of Kinesiology, The University of Toledo, Toledo, OH (United States)
  2. Centre d’Immunologie de Marseille-Luminy U2M, Aix-Marseille Université, Marseille (France)
  3. Department of Biological Sciences, The University of Toledo, Toledo, OH (United States)
Publication Date:
OSTI Identifier:
22462261
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research
Additional Journal Information:
Journal Volume: 331; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADHESION; ALIGNMENT; ANTIBODIES; ENGINEERING; FUNCTIONS; HYPERTROPHY; IN VITRO; INFLAMMATION; INHIBITION; MOLECULES; MYOBLASTS; PEPTIDES; PLANT GROWTH; REGENERATION; SIGNALS; SYNTHESIS

Citation Formats

Goh, Qingnian, Dearth, Christopher L., Corbett, Jacob T., Pierre, Philippe, INSERM U631, Institut National de la Santé et Recherche Médicale, Marseille, CNRS UMR6102, Centre National de la Recherche Scientifique, Marseille, Chadee, Deborah N., and Pizza, Francis X., E-mail: Francis.Pizza@utoledo.edu. Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis. United States: N. p., 2015. Web. doi:10.1016/J.YEXCR.2014.09.032.
Goh, Qingnian, Dearth, Christopher L., Corbett, Jacob T., Pierre, Philippe, INSERM U631, Institut National de la Santé et Recherche Médicale, Marseille, CNRS UMR6102, Centre National de la Recherche Scientifique, Marseille, Chadee, Deborah N., & Pizza, Francis X., E-mail: Francis.Pizza@utoledo.edu. Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis. United States. https://doi.org/10.1016/J.YEXCR.2014.09.032
Goh, Qingnian, Dearth, Christopher L., Corbett, Jacob T., Pierre, Philippe, INSERM U631, Institut National de la Santé et Recherche Médicale, Marseille, CNRS UMR6102, Centre National de la Recherche Scientifique, Marseille, Chadee, Deborah N., and Pizza, Francis X., E-mail: Francis.Pizza@utoledo.edu. 2015. "Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis". United States. https://doi.org/10.1016/J.YEXCR.2014.09.032.
@article{osti_22462261,
title = {Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis},
author = {Goh, Qingnian and Dearth, Christopher L. and Corbett, Jacob T. and Pierre, Philippe and INSERM U631, Institut National de la Santé et Recherche Médicale, Marseille and CNRS UMR6102, Centre National de la Recherche Scientifique, Marseille and Chadee, Deborah N. and Pizza, Francis X., E-mail: Francis.Pizza@utoledo.edu},
abstractNote = {We previously demonstrated that the expression of intercellular adhesion molecule-1 (ICAM-1) by skeletal muscle cells after muscle overload contributes to ensuing regenerative and hypertrophic processes in skeletal muscle. The objective of the present study is to reveal mechanisms through which skeletal muscle cell expression of ICAM-1 augments regenerative and hypertrophic processes of myogenesis. This was accomplished by genetically engineering C2C12 myoblasts to stably express ICAM-1, and by inhibiting the adhesive and signaling functions of ICAM-1 through the use of a neutralizing antibody or cell penetrating peptide, respectively. Expression of ICAM-1 by cultured skeletal muscle cells augmented myoblast–myoblast adhesion, myotube formation, myonuclear number, myotube alignment, myotube–myotube fusion, and myotube size without influencing the ability of myoblasts to proliferate or differentiate. ICAM-1 augmented myotube formation, myonuclear accretion, and myotube alignment through a mechanism involving adhesion-induced activation of ICAM-1 signaling, as these dependent measures were reduced via antibody and peptide inhibition of ICAM-1. The adhesive and signaling functions of ICAM-1 also facilitated myotube hypertrophy through a mechanism involving myotube–myotube fusion, protein synthesis, and Akt/p70s6k signaling. Our findings demonstrate that ICAM-1 expression by skeletal muscle cells augments myogenesis, and establish a novel mechanism through which the inflammatory response facilitates growth processes in skeletal muscle. - Highlights: • We examined mechanisms through which skeletal muscle cell expression of ICAM-1 facilitates events of in vitro myogenesis. • Expression of ICAM-1 by cultured myoblasts did not influence their ability to proliferate or differentiate. • Skeletal muscle cell expression of ICAM-1 augmented myoblast fusion, myotube alignment, myotube–myotube fusion, and myotube size. • ICAM-1 augmented myogenic processes through mechanisms involving its adhesive and signaling functions.},
doi = {10.1016/J.YEXCR.2014.09.032},
url = {https://www.osti.gov/biblio/22462261}, journal = {Experimental Cell Research},
issn = {0014-4827},
number = 2,
volume = 331,
place = {United States},
year = {Sun Feb 15 00:00:00 EST 2015},
month = {Sun Feb 15 00:00:00 EST 2015}
}