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Title: Acidic environment augments FcεRI-mediated production of IL-6 and IL-13 in mast cells

Although blood pH is maintained in a narrow range of around pH 7.4 in living organisms, inflammatory loci are characterized by acidic conditions. Mast cells tend to reside close to the surface of the body in areas such as the mucosa and skin where they may be exposed to exogenous acids, and they play an important role in immune responses. However, little is known about the effects of extracellular acidification on the functions of mast cell. Here, we found that extracellular acidification increased the dinitrophenyl-conjugated human serum albumin (DNP-HSA)-induced production of interleukin (IL)-6 and IL-13 in MC/9 cells or bone marrow-derived mouse mast cells sensitized with anti-DNP IgE. Extracellular acidification also inhibited migration of MC/9 cells toward DNP-HSA. In addition, acidic pH stimulated antigen-induced activation of p38 mitogen-activated protein kinase (MAPK) and protein kinase B (Akt). These findings suggest that extracellular acidification augmented antigen/IgE-induced and FcεRI-mediated production of IL-6 and IL-13 in mast cells, and that this was associated with the enhancement of p38 MAPK and Akt activation. - Highlights: • Antigen-induced IL-6 and IL-13 production was augmented by acidic pH in mast cells. • Acidic pH-induced actions were associated with activation of p38 MAPK and Akt. • Inhibition ofmore » p38 MAPK and Akt attenuated cytokine responses to acidic pH. • Acidic pH effects are not attributable to actions of known proton-sensing GPCRs.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [2] ; ;  [4] ;  [5] ; ; ;  [1] ;  [4] ;  [1] ;  [6] ;  [1] ;  [4]
  1. Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi (Japan)
  2. (Japan)
  3. Third Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui (Japan)
  4. Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi (Japan)
  5. Department of Anatomy, Graduate School of Medicine, Teikyo University, Tokyo (Japan)
  6. Gunma University Graduate School of Health Sciences, Maebashi (Japan)
Publication Date:
OSTI Identifier:
22462232
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 464; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACIDIFICATION; ALBUMINS; ANTIGENS; BLOOD SERUM; BONE MARROW; DINITROPHENOL; ENVIRONMENT; INFLAMMATION; INHIBITION; MAST CELLS; MICE; MUCOUS MEMBRANES; PH VALUE; PROTONS; SKIN; SURFACES