skip to main content

Title: Down-regulation of Rab5 decreases characteristics associated with maintenance of cell transformation

The early endosomal protein Rab5 is highly expressed in tumor samples, although a causal relationship between Rab5 expression and cell transformation has not been established. Here, we report the functional effects of targeting endogenous Rab5 with specific shRNA sequences in different tumor cell lines. Rab5 down-regulation in B16-F10 cells decreased tumor formation by subcutaneous injection into C57/BL6 mice. Accordingly, Rab5 targeting in B16-F10 and A549, but not MDA-MB-231 cells was followed by decreased cell proliferation, increased apoptosis and decreased anchorage-independent growth. These findings suggest that Rab5 expression is required to maintain characteristics associated with cell transformation. - Highlights: • Rab5 is important to the maintenance of cell transformation characteristics. • Down-regulation of Rab5 decreases cell proliferation and increases apoptosis in different cancer cells. • Rab5 is required for anchorage-independent growth and tumorigenicity in-vivo.
Authors:
;  [1] ;  [1] ;  [2] ;  [1] ;  [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [4]
  1. Institute for Research in Dental Sciences, Faculty of Dentistry, Universidad de Chile, Santiago (Chile)
  2. (ICBM), Faculty of Medicine, Universidad de Chile, Santiago (Chile)
  3. Center for Molecular Studies of the Cell, Institute of Biomedical Sciences (ICBM), Faculty of Medicine, Universidad de Chile, Santiago (Chile)
  4. (ACCDiS), Faculty of Medicine, Universidad de Chile, Santiago (Chile)
Publication Date:
OSTI Identifier:
22462216
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 464; Journal Issue: 2; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CELL PROLIFERATION; CELL TRANSFORMATIONS; IN VIVO; MAINTENANCE; MICE; NEOPLASMS; PLANT GROWTH; PROTEINS; SUBCUTANEOUS INJECTION; TRANSFORMATIONS; TUMOR CELLS; VIABILITY