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Title: A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures

Despite exciting new possibilities for regenerative therapy posed by the ability to induce pluripotent stem cells, recapitulation of three-dimensional kidneys for repair or replacement has not been possible. ARID3a-deficient mouse tissues generated multipotent, developmentally plastic cells. Therefore, we assessed the adult mouse ARID3a−/− kidney cell line, KKPS5, which expresses renal progenitor surface markers as an alternative cell source for modeling kidney development. Remarkably, these cells spontaneously developed into multicellular nephron-like structures in vitro, and engrafted into immunocompromised medaka mesonephros, where they formed mouse nephron structures. These data implicate KKPS5 cells as a new model system for studying kidney development. - Highlights: • An ARID3a-deficient mouse kidney cell line expresses multiple progenitor markers. • This cell line spontaneously forms multiple nephron-like structures in vitro. • This cell line formed mouse kidney structures in immunocompromised medaka fish kidneys. • Our data identify a novel model system for studying kidney development.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [1] ;  [1] ;  [1] ;  [1]
  1. Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK (United States)
  2. (United States)
  3. Immunobiology and Cancer Research, Oklahoma Medical Research Foundation, Oklahoma City, OK (United States)
Publication Date:
OSTI Identifier:
22462171
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 463; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADULTS; ANIMAL TISSUES; BIOLOGICAL REPAIR; IN VITRO; KIDNEYS; MICE; PLASTICS; STEM CELLS; SURFACES; THERAPY; THREE-DIMENSIONAL CALCULATIONS