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Title: Overexpression of Arabidopsis NADPH-dependent thioredoxin reductase C (AtNTRC) confers freezing and cold shock tolerance to plants

Overexpression of AtNTRC (AtNTRC{sup OE}) in Arabidopsis thaliana led to a freezing and cold stress tolerance, whereas a knockout mutant (atntrc) showed a stress-sensitive phenotype. Biochemical analyses showed that the recombinant AtNTRC proteins exhibited a cryoprotective activity for malate dehydrogenase and lactic dehydrogenase. Furthermore, conclusive evidence of its interaction with nucleic acids in vitro is provided here on the basis of gel shift and electron microscopy analysis. Recombinant AtNTRC efficiently protected RNA and DNA from RNase A and metal catalyzed oxidation damage, respectively. The C-terminal thioredoxin domain is required for the nucleic acid–protein complex formation. From these results, it can be hypothesized that AtNTRC, which is known to be an electron donor of peroxiredoxin, contributes the stability of macromolecules under cold stress. - Highlights: • AtNTRC has a cryoprotective activity in vitro. • Overexpression of AtNTRC increases tolerance to freezing and cold shock stresses. • Thioredoxin domain of AtNTRC protects nucleic acids in vitro. • AtNTRC inhibits protein aggregation under freezing stress in vitro.
Authors:
 [1] ;  [2] ;  [1] ; ;  [3] ;  [2] ;  [3] ;  [1] ;  [4] ;  [3]
  1. National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon-gun 325-813 (Korea, Republic of)
  2. Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon (Korea, Republic of)
  3. Division of Applied Life Science - BK21+ program, PMBBRC, Gyeongsang National University, Jinju (Korea, Republic of)
  4. (United States)
Publication Date:
OSTI Identifier:
22462169
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 463; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AGGLOMERATION; ARABIDOPSIS; DNA; ELECTRON MICROSCOPY; FREEZING; GELS; IN VITRO; METALS; OXIDATION; PHENOTYPE; PROTEINS; RNA; STRESSES; VALENCE