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Title: Hepassocin is required for hepatic outgrowth during zebrafish hepatogenesis

Background & aims: Hepassocin (HPS) is a hepatotrophic growth factor that specifically stimulates hepatocyte proliferation and promotes liver regeneration after liver damage. In this paper, zebrafish were used to investigate the role of HPS in liver development. Methods and results: During zebrafish development, HPS expression is enriched in liver throughout hepatogenesis. Knockdown of HPS using its specific morpholino leads to a smaller liver phenotype. Further results showed that the HPS knockdown has no effect on the expression of the early endoderm marker gata6 and early hepatic marker hhex. In addition, results showed that the smaller-liver phenotype in HPS morphants was caused by suppression of cell proliferation, not induction of cell apoptosis. Conclusions: Current findings indicated that HPS is essential to the later stages of development in vertebrate liver organogenesis. - Highlights: • HPS is enriched in zebrafish liver and has strong similarities with other species. • Knocking down HPS with MOs results in small liver phenotype. • HPS depletion regulates liver outgrowth but not liver specification and budding. • HPS depletion causes hepatocyte proliferation arrest but not apoptosis induction.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [2] ;  [5] ; ; ; ; ;  [4] ;  [2] ;  [1] ;  [1] ;  [2] ;  [2]
  1. Tianjin University, Department of Pharmaceutical Engineering, Tianjin 300072 (China)
  2. (China)
  3. Beijing Institute of Pharmacology and Toxicology, Beijing 100850 (China)
  4. Beijing Institute of Radiation Medicine, Beijing 100850 (China)
  5. Institute of Zoology, Chinese Academy of Sciences, Beijing 100101 (China)
Publication Date:
OSTI Identifier:
22462129
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 463; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; CARBOXYLIC ACIDS; CELL PROLIFERATION; DAMAGE; FIBRINOGEN; GROWTH FACTORS; HEPATOMAS; INHIBITION; LIVER; PHENOTYPE; SILICON OXIDES; SPECIFICATIONS; VERTEBRATES