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Title: PPARγ inhibits ovarian cancer cells proliferation through upregulation of miR-125b

miR-125b has essential roles in coordinating tumor proliferation, angiogenesis, invasiveness, metastasis and chemotherapy recurrence. In ovarian cancer miR-125b has been shown to be downregulated and acts as a tumor suppressor by targeting proto-oncogene BCL3. PPARγ, a multiple functional transcription factor, has been reported to have anti-tumor effects through inhibition of proliferation and induction of differentiation and apoptosis by targeting the tumor related genes. However, it is unclear whether miR-125b is regulated by PPARγ in ovarian cancer. In this study, we demonstrated that the miR-125b downregulated in ovarian cancer tissues and cell lines. Ligands-activated PPARγ suppressed proliferation of ovarian cancer cells and this PPARγ-induced growth inhibition is mediated by the upregulation of miR-125b. PPARγ promoted the expression of miR-125b by directly binding to the responsive element in miR-125b gene promoter region. Thus, our results suggest that PPARγ can induce growth suppression of ovarian cancer by upregulating miR-125b which inhibition of proto-oncogene BCL3. These findings will extend our understanding of the function of PPARγ in tumorigenesis and miR-125b may be a therapeutic intervention of ovarian cancer. - Highlights: • miR-125b is down-regulated in ovarian cancer tissues and cells. • PPARγ upregulates miR-125b and downregulates its target gene BCL3 expression. • Silence ofmore » miR-125b attenuates PPARγ-mediated growth suppression of ovarian cancer cells. • PPARγ promotes the transcription of miR-125b via binding to PPARE in miR-125b gene promoter region.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5]
  1. Department of Obstetrics and Gynecology, Suining Central Hospital, Suining (China)
  2. Department of Gynecology and Obsterics, Jinan Central Hospital, Jinan (China)
  3. Department of Otorhinolaryngolgy, Suining Central Hospital, Suining (China)
  4. Department of Gynecology and Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing (China)
  5. Department of Gynecology and Obsterics, Zhongshan Hospital, Wuhan (China)
Publication Date:
OSTI Identifier:
22462090
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 462; Journal Issue: 2; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADMINISTRATIVE PROCEDURES; ANGIOGENESIS; ANIMAL TISSUES; APOPTOSIS; CELL PROLIFERATION; CHEMOTHERAPY; COORDINATES; INHIBITION; LIGANDS; METASTASES; NEOPLASMS; ONCOGENES; OVARIES; PLANT GROWTH; PROMOTERS; TRANSCRIPTION; TRANSCRIPTION FACTORS