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Title: Effective delivery of recombinant proteins to rod photoreceptors via lipid nanovesicles

The potential of liposomes to deliver functional proteins in retinal photoreceptors and modulate their physiological response was investigated by two experimental approaches. First, we treated isolated mouse retinas with liposomes encapsulating either recoverin, an important endogenous protein operating in visual phototransduction, or antibodies against recoverin. We then intravitrally injected in vivo liposomes encapsulating either rhodamin B or recoverin and we investigated the distribution in retina sections by confocal microscopy. The content of liposomes was found to be released in higher amount in the photoreceptor layer than in the other regions of the retina and the functional effects of the release were in line with the current model of phototransduction. Our study sets the basis for quantitative investigations aimed at assessing the potential of intraocular protein delivery via biocompatible nanovesicles, with promising implications for the treatment of retinal diseases affecting the photoreceptor layer. - Highlights: • Recombinant proteins encapsulated in nano-sized liposomes injected intravitreally reach retinal photoreceptors. • The phototransduction cascade in rods is modulated by the liposome content. • Mathematical modeling predicts the alteration of the photoresponses following liposome fusion.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [3] ;  [4] ;  [2] ;  [4]
  1. Dept. of Translational Research, University of Pisa, Pisa (Italy)
  2. Dept. of Life Sciences and Reproduction, University of Verona, Strada Le Grazie 8, Verona (Italy)
  3. Dept. of Neurological and Movement Sciences, University of Verona, Strada Le Grazie 8, Verona (Italy)
  4. (Italy)
Publication Date:
OSTI Identifier:
22462081
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 461; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; DELIVERY; DISEASES; DISTRIBUTION; IN VIVO; LAYERS; LIPIDS; LIPOSOMES; MATHEMATICAL MODELS; MICE; MICROSCOPY; NANOSTRUCTURES; PROTEINS; RETINA; RODS; THERAPY