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Title: Stronger activation of SREBP-1a by nucleus-localized HBx

We previously showed that hepatitis B virus (HBV) X protein activates the sterol regulatory element-binding protein-1a (SREBP-1a). Here we examined the role of nuclear localization of HBx in this process. In comparison to the wild-type and cytoplasmic HBx, nuclear HBx had stronger effects on SREBP-1a and fatty acid synthase transcription activation, intracellular lipid accumulation and cell proliferation. Furthermore, nuclear HBx could activate HBV enhancer I/X promoter and was more effective on up-regulating HBV mRNA level in the context of HBV replication than the wild-type HBx, while the cytoplasmic HBx had no effect. Our results demonstrate the functional significance of the nucleus-localized HBx in regulating host lipogenic pathway and HBV replication. - Highlights: • Nuclear HBx is more effective on activating SREBP-1a and FASN transcription. • Nuclear HBx is more effective on enhancing intracellular lipid accumulation. • Nuclear HBx is more effective on enhancing cell proliferation. • Nuclear HBx up-regulates HBV enhancer I/X promoter activity. • Nuclear HBx increases HBV mRNA level in the context of HBV replication.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [4]
  1. VIDO-InterVac, Veterinary Microbiology, University of Saskatchewan, Saskatoon (Canada)
  2. VIDO-InterVac, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)
  3. Drug Discovery Group, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)
  4. VIDO-InterVac, Veterinary Microbiology, Vaccinology and Immunotherapeutics, University of Saskatchewan, Saskatoon, Saskatchewan (Canada)
Publication Date:
OSTI Identifier:
22462032
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 460; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BUILDUP; CARBOXYLIC ACIDS; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; HEPATITIS; LIPIDS; MESSENGER-RNA; PROMOTERS; PROTEINS; STEROLS; TRANSCRIPTION; VIRUSES