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Title: Regulation of retinoid X receptor gamma expression by fed state in mouse liver

Abstract

Glucose metabolism is balanced by glycolysis and gluconeogenesis with precise control in the liver. The expression of genes related to glucose metabolism is regulated primarily by glucose and insulin at transcriptional level. Nuclear receptors play important roles in regulating the gene expression of glucose metabolism at transcriptional level. Some of these nuclear receptors form heterodimers with RXRs to bind to their specific regulatory elements on the target promoters. To date, three isotypes of RXRs have been identified; RXRα, RXRβ and RXRγ. However, their involvement in the interactions with other nuclear receptors in the liver remains unclear. In this study, we found RXRγ is rapidly induced after feeding in the mouse liver, indicating a potential role of RXRγ in controlling glucose or lipid metabolism in the fasting–feeding cycle. In addition, RXRγ expression was upregulated by glucose in primary hepatocytes. This implies that glucose metabolism governed by RXRγ in conjunction with other nuclear receptors. The luciferase reporter assay showed that RXRγ as well as RXRα increased SREBP-1c promoter activity in hepatocytes. These results suggest that RXRγ may play an important role in tight control of glucose metabolism in the fasting–feeding cycle. - Highlights: • Refeeding increases the RXRγ expression level in mousemore » liver. • RXRγ expression is induced by high glucose condition in primary hepatocytes. • RXRγ and LXRα have synergistic effect on SREBP-1c promoter activity. • RXRγ binds to LXRE(-299/-280) located within SREBP-1c promoter region and interacts with LXRα.« less

Authors:
 [1];  [2];  [3];  [3]
  1. Department of Biochemistry, College of Medicine, Catholic Kwandong University, Gangneung 210-701 (Korea, Republic of)
  2. Division of Metabolic Disease, Center for Biomedical Sciences, National Institute of Health Korea, Osong 361-709 (Korea, Republic of)
  3. Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul 120-752 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22458506
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 458; Journal Issue: 1; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADIPOSE TISSUE; FASTING; FEEDING; GENES; GLUCOSE; GLYCOLYSIS; INSULIN; LIPIDS; LIVER; LIVER CELLS; LUCIFERASE; MICE; PHOSPHOENOLPYRUVATE; PROMOTERS; RECEPTORS; RETINOIC ACID; STEROLS; TRANSCRIPTION

Citation Formats

Park, Sangkyu, Lee, Yoo Jeong, Ko, Eun Hee, Kim, Jae-woo, and Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul 120-752. Regulation of retinoid X receptor gamma expression by fed state in mouse liver. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.01.082.
Park, Sangkyu, Lee, Yoo Jeong, Ko, Eun Hee, Kim, Jae-woo, & Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul 120-752. Regulation of retinoid X receptor gamma expression by fed state in mouse liver. United States. https://doi.org/10.1016/J.BBRC.2015.01.082
Park, Sangkyu, Lee, Yoo Jeong, Ko, Eun Hee, Kim, Jae-woo, and Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul 120-752. 2015. "Regulation of retinoid X receptor gamma expression by fed state in mouse liver". United States. https://doi.org/10.1016/J.BBRC.2015.01.082.
@article{osti_22458506,
title = {Regulation of retinoid X receptor gamma expression by fed state in mouse liver},
author = {Park, Sangkyu and Lee, Yoo Jeong and Ko, Eun Hee and Kim, Jae-woo and Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul 120-752},
abstractNote = {Glucose metabolism is balanced by glycolysis and gluconeogenesis with precise control in the liver. The expression of genes related to glucose metabolism is regulated primarily by glucose and insulin at transcriptional level. Nuclear receptors play important roles in regulating the gene expression of glucose metabolism at transcriptional level. Some of these nuclear receptors form heterodimers with RXRs to bind to their specific regulatory elements on the target promoters. To date, three isotypes of RXRs have been identified; RXRα, RXRβ and RXRγ. However, their involvement in the interactions with other nuclear receptors in the liver remains unclear. In this study, we found RXRγ is rapidly induced after feeding in the mouse liver, indicating a potential role of RXRγ in controlling glucose or lipid metabolism in the fasting–feeding cycle. In addition, RXRγ expression was upregulated by glucose in primary hepatocytes. This implies that glucose metabolism governed by RXRγ in conjunction with other nuclear receptors. The luciferase reporter assay showed that RXRγ as well as RXRα increased SREBP-1c promoter activity in hepatocytes. These results suggest that RXRγ may play an important role in tight control of glucose metabolism in the fasting–feeding cycle. - Highlights: • Refeeding increases the RXRγ expression level in mouse liver. • RXRγ expression is induced by high glucose condition in primary hepatocytes. • RXRγ and LXRα have synergistic effect on SREBP-1c promoter activity. • RXRγ binds to LXRE(-299/-280) located within SREBP-1c promoter region and interacts with LXRα.},
doi = {10.1016/J.BBRC.2015.01.082},
url = {https://www.osti.gov/biblio/22458506}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 458,
place = {United States},
year = {Fri Feb 27 00:00:00 EST 2015},
month = {Fri Feb 27 00:00:00 EST 2015}
}