miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma
Abstract
microRNAs (miRNAs) are frequently dysregulated in human malignancies. It was recently shown that miR-204-5p is downregulated in papillary thyroid carcinoma (PTC); however, the functional significance of this observation is not known. This study investigated the role of miR-204-5p in PTC. Overexpressing miR-204-5p suppressed PTC cell proliferation and induced cell cycle arrest and apoptosis. The results of a luciferase reporter assay showed that miR-204-5p can directly bind to the 3′ untranslated region (UTR) of insulin-like growth factor-binding protein 5 (IGFBP5) mRNA, and IGFBP5 overexpression partially reversed the growth-inhibitory effects of miR-204-5p. These results indicate that miR-204-5p acts as a tumor suppressor in PTC by regulating IGFBP5 expression and that miR-204-5p can potentially serve as an antitumorigenic agent in the treatment of PTC. - Highlights: • miR-204-5p expression is downregulated in PTC tissues and cell lines. • miR-204-5p suppresses proliferation and promotes apoptosis in PTC cells. • miR-204-5p suppresses IGFBP5 expression by direct binding to the 3′-UTR. • IGFBP5 overexpression reverses the effects of miR-204-5p.
- Authors:
- Publication Date:
- OSTI Identifier:
- 22458492
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 457; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; APOPTOSIS; CARCINOMAS; CELL CYCLE; CELL PROLIFERATION; GROWTH FACTORS; INSULIN; LUCIFERASE; MESSENGER-RNA; PLANT GROWTH; THYROID
Citation Formats
Liu, Lianyong, Wang, Jingnan, Li, Xiangqi, Ma, Junhua, Shi, Chao, Zhu, Hongling, Xi, Qian, Zhang, Jichen, Zhao, Xuemei, and Gu, Mingjun. miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma. United States: N. p., 2015.
Web. doi:10.1016/J.BBRC.2015.01.037.
Liu, Lianyong, Wang, Jingnan, Li, Xiangqi, Ma, Junhua, Shi, Chao, Zhu, Hongling, Xi, Qian, Zhang, Jichen, Zhao, Xuemei, & Gu, Mingjun. miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma. United States. https://doi.org/10.1016/J.BBRC.2015.01.037
Liu, Lianyong, Wang, Jingnan, Li, Xiangqi, Ma, Junhua, Shi, Chao, Zhu, Hongling, Xi, Qian, Zhang, Jichen, Zhao, Xuemei, and Gu, Mingjun. 2015.
"miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma". United States. https://doi.org/10.1016/J.BBRC.2015.01.037.
@article{osti_22458492,
title = {miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma},
author = {Liu, Lianyong and Wang, Jingnan and Li, Xiangqi and Ma, Junhua and Shi, Chao and Zhu, Hongling and Xi, Qian and Zhang, Jichen and Zhao, Xuemei and Gu, Mingjun},
abstractNote = {microRNAs (miRNAs) are frequently dysregulated in human malignancies. It was recently shown that miR-204-5p is downregulated in papillary thyroid carcinoma (PTC); however, the functional significance of this observation is not known. This study investigated the role of miR-204-5p in PTC. Overexpressing miR-204-5p suppressed PTC cell proliferation and induced cell cycle arrest and apoptosis. The results of a luciferase reporter assay showed that miR-204-5p can directly bind to the 3′ untranslated region (UTR) of insulin-like growth factor-binding protein 5 (IGFBP5) mRNA, and IGFBP5 overexpression partially reversed the growth-inhibitory effects of miR-204-5p. These results indicate that miR-204-5p acts as a tumor suppressor in PTC by regulating IGFBP5 expression and that miR-204-5p can potentially serve as an antitumorigenic agent in the treatment of PTC. - Highlights: • miR-204-5p expression is downregulated in PTC tissues and cell lines. • miR-204-5p suppresses proliferation and promotes apoptosis in PTC cells. • miR-204-5p suppresses IGFBP5 expression by direct binding to the 3′-UTR. • IGFBP5 overexpression reverses the effects of miR-204-5p.},
doi = {10.1016/J.BBRC.2015.01.037},
url = {https://www.osti.gov/biblio/22458492},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 457,
place = {United States},
year = {Fri Feb 20 00:00:00 EST 2015},
month = {Fri Feb 20 00:00:00 EST 2015}
}