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Title: Developmental programming: Prenatal BPA treatment disrupts timing of LH surge and ovarian follicular wave dynamics in adult sheep

Abstract

Developmental exposure to BPA adversely affects reproductive function. In sheep, prenatal BPA treatment induces reproductive neuroendocrine defects, manifested as LH excess and dampened LH surge and perturbs early ovarian gene expression. In this study we hypothesized that prenatal BPA treatment will also disrupt ovarian follicular dynamics. Pregnant sheep were treated from days 30 to 90 of gestation with 3 different BPA doses (0.05, 0.5, or 5 mg/kg BW/day). All female offspring were estrus synchronized and transrectal ultrasonography was performed daily for 22 days to monitor ovarian follicular and corpora lutea dynamics. Blood samples were collected to assess preovulatory hormonal changes and luteal progesterone dynamics. Statistical analysis revealed that the time interval between the estradiol rise and the preovulatory LH surge was shortened in the BPA-treated females. None of the three BPA doses had an effect on corpora lutea, progestogenic cycles, and mean number or duration of ovulatory and non-ovulatory follicles. However, differences in follicular count trajectories were evident in all three follicular size classes (2–3 mm, 4–5 mm, and ≥ 6 mm) of prenatal BPA-treated animals compared to controls. Number of follicular waves tended also to be more variable in the prenatal BPA-treated groups ranging from 2 to 5 follicularmore » waves per cycle, while this was restricted to 3 to 4 waves in control females. These changes in ovarian follicular dynamics coupled with defects in time interval between estradiol rise and preovulatory LH release are likely to lead to subfertility in prenatal BPA-treated females. - Highlights: • Prenatal BPA shortens interval between estradiol rise and preovulatory LH surge. • Prenatal BPA affects follicular count trajectory and follicular wave occurrence. • Prenatal BPA does not affect ovulatory rate and progesterone dynamics.« less

Authors:
; ;  [1];  [2]
  1. Department of Pediatrics, University of Michigan, Ann Arbor, MI (United States)
  2. Department of Biostatistics, University of Michigan, Ann Arbor, MI (United States)
Publication Date:
OSTI Identifier:
22439811
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 279; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BLOOD; BONNEVILLE POWER ADMINISTRATION; DOSES; ESTRADIOL; FEMALES; GENES; LIBERINS; LUTEINIZING HORMONE; OVARIES; PROGENY; PROGESTERONE; PROSTAGLANDINS; RECEPTORS; SHEEP; ULTRASONOGRAPHY

Citation Formats

Veiga-Lopez, A., Beckett, E. M., Abi Salloum, B., Ye, W., Padmanabhan, V., E-mail: vasantha@umich.edu, and The Reproductive Sciences Program, University of Michigan, Ann Arbor, MI. Developmental programming: Prenatal BPA treatment disrupts timing of LH surge and ovarian follicular wave dynamics in adult sheep. United States: N. p., 2014. Web. doi:10.1016/J.TAAP.2014.05.016.
Veiga-Lopez, A., Beckett, E. M., Abi Salloum, B., Ye, W., Padmanabhan, V., E-mail: vasantha@umich.edu, & The Reproductive Sciences Program, University of Michigan, Ann Arbor, MI. Developmental programming: Prenatal BPA treatment disrupts timing of LH surge and ovarian follicular wave dynamics in adult sheep. United States. https://doi.org/10.1016/J.TAAP.2014.05.016
Veiga-Lopez, A., Beckett, E. M., Abi Salloum, B., Ye, W., Padmanabhan, V., E-mail: vasantha@umich.edu, and The Reproductive Sciences Program, University of Michigan, Ann Arbor, MI. 2014. "Developmental programming: Prenatal BPA treatment disrupts timing of LH surge and ovarian follicular wave dynamics in adult sheep". United States. https://doi.org/10.1016/J.TAAP.2014.05.016.
@article{osti_22439811,
title = {Developmental programming: Prenatal BPA treatment disrupts timing of LH surge and ovarian follicular wave dynamics in adult sheep},
author = {Veiga-Lopez, A. and Beckett, E. M. and Abi Salloum, B. and Ye, W. and Padmanabhan, V., E-mail: vasantha@umich.edu and The Reproductive Sciences Program, University of Michigan, Ann Arbor, MI},
abstractNote = {Developmental exposure to BPA adversely affects reproductive function. In sheep, prenatal BPA treatment induces reproductive neuroendocrine defects, manifested as LH excess and dampened LH surge and perturbs early ovarian gene expression. In this study we hypothesized that prenatal BPA treatment will also disrupt ovarian follicular dynamics. Pregnant sheep were treated from days 30 to 90 of gestation with 3 different BPA doses (0.05, 0.5, or 5 mg/kg BW/day). All female offspring were estrus synchronized and transrectal ultrasonography was performed daily for 22 days to monitor ovarian follicular and corpora lutea dynamics. Blood samples were collected to assess preovulatory hormonal changes and luteal progesterone dynamics. Statistical analysis revealed that the time interval between the estradiol rise and the preovulatory LH surge was shortened in the BPA-treated females. None of the three BPA doses had an effect on corpora lutea, progestogenic cycles, and mean number or duration of ovulatory and non-ovulatory follicles. However, differences in follicular count trajectories were evident in all three follicular size classes (2–3 mm, 4–5 mm, and ≥ 6 mm) of prenatal BPA-treated animals compared to controls. Number of follicular waves tended also to be more variable in the prenatal BPA-treated groups ranging from 2 to 5 follicular waves per cycle, while this was restricted to 3 to 4 waves in control females. These changes in ovarian follicular dynamics coupled with defects in time interval between estradiol rise and preovulatory LH release are likely to lead to subfertility in prenatal BPA-treated females. - Highlights: • Prenatal BPA shortens interval between estradiol rise and preovulatory LH surge. • Prenatal BPA affects follicular count trajectory and follicular wave occurrence. • Prenatal BPA does not affect ovulatory rate and progesterone dynamics.},
doi = {10.1016/J.TAAP.2014.05.016},
url = {https://www.osti.gov/biblio/22439811}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 2,
volume = 279,
place = {United States},
year = {Mon Sep 01 00:00:00 EDT 2014},
month = {Mon Sep 01 00:00:00 EDT 2014}
}