Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats
Abstract
We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh β, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T{sub 3}-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T{sub 4} bound to Tg, determined thyroid T{sub 4} levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T{sub 4} levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05)more »
- Authors:
-
- Institute for Biological Research, Siniša Stanković, University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade (Serbia)
- Institut für Experimentelle Endokrinologie, Charité — Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin (Germany)
- Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000 Niš (Serbia)
- Institute for Science Application in Agriculture, University of Belgrade, Despot Stefan Blvd. 68b, 11000 Belgrade (Serbia)
- Publication Date:
- OSTI Identifier:
- 22439784
- Resource Type:
- Journal Article
- Journal Name:
- Toxicology and Applied Pharmacology
- Additional Journal Information:
- Journal Volume: 278; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CONCENTRATION RATIO; ENZYME ACTIVITY; GENES; GROWTH FACTORS; HOMEOSTASIS; KIDNEYS; LIVER; RATS; RECEPTORS; SYNTHESIS; THYROGLOBULIN; THYROID; THYROID HORMONES; TRH; TSH
Citation Formats
Šošić-Jurjević, Branka, Filipović, Branko, Wirth, Eva Katrin, Živanović, Jasmina, Radulović, Niko, Janković, Snežana, Milošević, Verica, and Köhrle, Josef. Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats. United States: N. p., 2014.
Web. doi:10.1016/J.TAAP.2014.04.018.
Šošić-Jurjević, Branka, Filipović, Branko, Wirth, Eva Katrin, Živanović, Jasmina, Radulović, Niko, Janković, Snežana, Milošević, Verica, & Köhrle, Josef. Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats. United States. https://doi.org/10.1016/J.TAAP.2014.04.018
Šošić-Jurjević, Branka, Filipović, Branko, Wirth, Eva Katrin, Živanović, Jasmina, Radulović, Niko, Janković, Snežana, Milošević, Verica, and Köhrle, Josef. 2014.
"Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats". United States. https://doi.org/10.1016/J.TAAP.2014.04.018.
@article{osti_22439784,
title = {Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats},
author = {Šošić-Jurjević, Branka and Filipović, Branko and Wirth, Eva Katrin and Živanović, Jasmina and Radulović, Niko and Janković, Snežana and Milošević, Verica and Köhrle, Josef},
abstractNote = {We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh β, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T{sub 3}-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T{sub 4} bound to Tg, determined thyroid T{sub 4} levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T{sub 4} levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05) increased. At the level of pituitary, no significant change in gene expression levels, or Dio 1 and 2 enzyme activities was observed. In conclusion, both G and D impaired Tg and TH synthesis, but at the same time increased tissue availability of TH in peripheral tissues of Orx middle-aged rats. - Highlights: • We tested how genistein and daidzein interfere with thyroid hormone homeostasis. • Thyroid: decreased expression of Tg and TPO genes correlated with IHC results. • Serum: total T{sub 4} reduced and TSH increased. • Liver and kidney: expression of Spot 14 and liver Dio 1 activity increased. • Pituitary: expression of T{sub 3}-regulated genes and Dio 1 and 2 activities unchanged.},
doi = {10.1016/J.TAAP.2014.04.018},
url = {https://www.osti.gov/biblio/22439784},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 2,
volume = 278,
place = {United States},
year = {Tue Jul 15 00:00:00 EDT 2014},
month = {Tue Jul 15 00:00:00 EDT 2014}
}