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Title: Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats

Abstract

We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh β, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T{sub 3}-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T{sub 4} bound to Tg, determined thyroid T{sub 4} levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T{sub 4} levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05)more » increased. At the level of pituitary, no significant change in gene expression levels, or Dio 1 and 2 enzyme activities was observed. In conclusion, both G and D impaired Tg and TH synthesis, but at the same time increased tissue availability of TH in peripheral tissues of Orx middle-aged rats. - Highlights: • We tested how genistein and daidzein interfere with thyroid hormone homeostasis. • Thyroid: decreased expression of Tg and TPO genes correlated with IHC results. • Serum: total T{sub 4} reduced and TSH increased. • Liver and kidney: expression of Spot 14 and liver Dio 1 activity increased. • Pituitary: expression of T{sub 3}-regulated genes and Dio 1 and 2 activities unchanged.« less

Authors:
 [1];  [1];  [2];  [1];  [3];  [4];  [1];  [2]
  1. Institute for Biological Research, Siniša Stanković, University of Belgrade, Despot Stefan Blvd. 142, 11000 Belgrade (Serbia)
  2. Institut für Experimentelle Endokrinologie, Charité — Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin (Germany)
  3. Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000 Niš (Serbia)
  4. Institute for Science Application in Agriculture, University of Belgrade, Despot Stefan Blvd. 68b, 11000 Belgrade (Serbia)
Publication Date:
OSTI Identifier:
22439784
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 278; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CONCENTRATION RATIO; ENZYME ACTIVITY; GENES; GROWTH FACTORS; HOMEOSTASIS; KIDNEYS; LIVER; RATS; RECEPTORS; SYNTHESIS; THYROGLOBULIN; THYROID; THYROID HORMONES; TRH; TSH

Citation Formats

Šošić-Jurjević, Branka, Filipović, Branko, Wirth, Eva Katrin, Živanović, Jasmina, Radulović, Niko, Janković, Snežana, Milošević, Verica, and Köhrle, Josef. Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats. United States: N. p., 2014. Web. doi:10.1016/J.TAAP.2014.04.018.
Šošić-Jurjević, Branka, Filipović, Branko, Wirth, Eva Katrin, Živanović, Jasmina, Radulović, Niko, Janković, Snežana, Milošević, Verica, & Köhrle, Josef. Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats. United States. https://doi.org/10.1016/J.TAAP.2014.04.018
Šošić-Jurjević, Branka, Filipović, Branko, Wirth, Eva Katrin, Živanović, Jasmina, Radulović, Niko, Janković, Snežana, Milošević, Verica, and Köhrle, Josef. 2014. "Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats". United States. https://doi.org/10.1016/J.TAAP.2014.04.018.
@article{osti_22439784,
title = {Soy isoflavones interfere with thyroid hormone homeostasis in orchidectomized middle-aged rats},
author = {Šošić-Jurjević, Branka and Filipović, Branko and Wirth, Eva Katrin and Živanović, Jasmina and Radulović, Niko and Janković, Snežana and Milošević, Verica and Köhrle, Josef},
abstractNote = {We previously reported that genistein (G) and daidzein (D) administered subcutaneously (10 mg/kg) induce changes in the angio-follicular units of the thyroid gland, reduce concentration of total thyroid hormones (TH) and increase thyrotropin (TSH) in serum of orchidectomized middle-aged (16-month-old) rats. To further investigate these effects, we now examined expression levels of the thyroglobulin (Tg), thyroperoxidase (Tpo), vascular endothelial growth factor A (Vegfa) and deiodinase type 1 (Dio 1) genes in the thyroid; in the pituitary, genes involved in TH feedback control (Tsh β, Dio 1, Dio 2, Trh receptor); and in the liver and kidney, expression of T{sub 3}-activated genes Dio 1 and Spot 14, as well as transthyretin (Ttr), by quantitative real-time PCR. We also analyzed TPO-immunopositivity and immunofluorescence of T{sub 4} bound to Tg, determined thyroid T{sub 4} levels and measured deiodinase enzyme activities in examined organs. Decreased expression of Tg and Tpo genes (p < 0.05) correlated with immunohistochemical staining results, and together with decreased serum total T{sub 4} levels, indicates decreased Tg and TH synthesis following treatments with both isoflavones. However, expression of Spot 14 (p < 0.05) gene in liver and kidney was up-regulated, and liver Dio 1 expression and activity (p < 0.05) increased. At the level of pituitary, no significant change in gene expression levels, or Dio 1 and 2 enzyme activities was observed. In conclusion, both G and D impaired Tg and TH synthesis, but at the same time increased tissue availability of TH in peripheral tissues of Orx middle-aged rats. - Highlights: • We tested how genistein and daidzein interfere with thyroid hormone homeostasis. • Thyroid: decreased expression of Tg and TPO genes correlated with IHC results. • Serum: total T{sub 4} reduced and TSH increased. • Liver and kidney: expression of Spot 14 and liver Dio 1 activity increased. • Pituitary: expression of T{sub 3}-regulated genes and Dio 1 and 2 activities unchanged.},
doi = {10.1016/J.TAAP.2014.04.018},
url = {https://www.osti.gov/biblio/22439784}, journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 2,
volume = 278,
place = {United States},
year = {Tue Jul 15 00:00:00 EDT 2014},
month = {Tue Jul 15 00:00:00 EDT 2014}
}