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Title: Death-domain associated protein-6 (DAXX) mediated apoptosis in hantavirus infection is counter-balanced by activation of interferon-stimulated nuclear transcription factors

Hantaviruses are negative strand RNA species that replicate predominantly in the cytoplasm. They also activate numerous cellular responses, but their involvement in nuclear processes is yet to be established. Using human umbilical vein endothelial cells (HUVECs), this study investigates the molecular finger-print of nuclear transcription factors during hantavirus infection. The viral-replication-dependent activation of pro-myelocytic leukemia protein (PML) was followed by subsequent localization in nuclear bodies (NBs). PML was also found in close proximity to activated Sp100 nuclear antigen and interferon-stimulated gene 20 kDa protein (ISG-20), but co-localization with death-domain associated protein-6 (DAXX) was not observed. These data demonstrate that hantavirus triggers PML activation and localization in NBs in the absence of DAXX-PLM-NB co-localization. The results suggest that viral infection interferes with DAXX-mediated apoptosis, and expression of interferon-activated Sp100 and ISG-20 proteins may indicate intracellular intrinsic antiviral attempts.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [1] ;  [6]
  1. Whittemore Peterson Institute, University of Nevada-Reno, Reno (United States)
  2. Department of Pathology and Nevada State Health Laboratory, University of Nevada-Reno, Reno (United States)
  3. Kazan State Medical University, Kazan (Russian Federation)
  4. Asklepios-Med (private medical practice and research center), Szeged (Hungary)
  5. Department of Microbiology and Immunology, University of Nevada-Reno, Reno (United States)
  6. Department of Genetics, Kazan (Volga Region) Federal University, Kazan (Russian Federation)
Publication Date:
OSTI Identifier:
22436639
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 443; Journal Issue: 2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; APOPTOSIS; BALANCES; CYTOPLASM; DEATH; GENES; INTERFERON; LEUKEMIA; RNA; TRANSCRIPTION FACTORS; VEINS