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Title: Attenuation of virus production at high multiplicities of infection in Aureococcus anophagefferens

Infection dynamics (saturation kinetics, infection efficiency, adsorption and burst size) for the Aureococcus anophagefferens-Brown Tide virus (AaV) system were investigated using susceptible and resistant strains. Adsorption assays revealed that virus affinity to the cell surface is a key determinant of infectivity. Saturation of infection occurred at a multiplicity of infection (MOI) of 8 viruses per host and resulted in ∼90–95% of infected cells, with burst sizes ranging from 164 to 191. Insight from the AaV genome implicates recycling of host nucleotides rather than de novo synthesis as a constraint on viral replication. Viral yields and mean burst sizes were significantly diminished with increasing MOI. This phenomenon, which was reminiscent of phage-induced ‘lysis from without’, appeared to be caused by viral contact and was unrelated to bacteria, signaling/toxic compounds, or defective interfering viruses. We posit that high-MOI effects attenuate viral proliferation in natural systems providing a negative feedback on virus-induced bloom collapse.
Authors:
;
Publication Date:
OSTI Identifier:
22435060
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 466-467; Conference: 1. international symposium on giant virus biology, Ringsberg (Germany), 17-20 Nov 2013; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADSORPTION; AFFINITY; BACTERIA; BACTERIOPHAGES; CELL PROLIFERATION; EDTA; EFFICIENCY; ENZYMES; HOST; INFECTIVITY; LYSIS; NUCLEOTIDES; PHYTOPLANKTON; PRODUCTIVITY; SALINITY; SIGNALS; STRAINS; SYNTHESIS; TOXICITY