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Title: Initial characterization of Vaccinia Virus B4 suggests a role in virus spread

Currently, little is known about the ankyrin/F-box protein B4. Here, we report that B4R-null viruses exhibited reduced plaque size in tissue culture, and decreased ability to spread, as assessed by multiple-step growth analysis. Electron microscopy indicated that B4R-null viruses still formed mature and extracellular virions; however, there was a slight decrease of virions released into the media following deletion of B4R. Deletion of B4R did not affect the ability of the virus to rearrange actin; however, VACV811, a large vaccinia virus deletion mutant missing 55 open reading frames, had decreased ability to produce actin tails. Using ectromelia virus, a natural mouse pathogen, we demonstrated that virus devoid of EVM154, the B4R homolog, showed decreased spread to organs and was attenuated during infection. This initial characterization suggests that B4 may play a role in virus spread, and that other unidentified mediators of actin tail formation may exist in vaccinia virus. - Highlights: • B4R-null viruses show reduced plaque size, and decreased ability to spread. • B4R-null viruses formed mature and extracellular virions; and rearranged actin. • Virus devoid of EVM154, the B4R homolog, was attenuated during infection. • Initial characterization suggests that B4 may play a role in virus spread. •more » Unidentified mediators of actin tail formation may exist in vaccinia virus.« less
Authors:
; ;  [1] ;  [2] ;  [1] ;  [2] ;  [1]
  1. Li Ka Shing Institute of Virology, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada T6G 2S2 (Canada)
  2. Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, MO (United States)
Publication Date:
OSTI Identifier:
22435034
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 456-457; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; ELECTRON MICROSCOPY; MICE; MUTANTS; ORGANS; PATHOGENS; PLANT GROWTH; TISSUE CULTURES; VACCINIA VIRUS; VIRULENCE