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Title: Identification and characterization of multiple conserved nuclear localization signals within adenovirus E1A

The human adenovirus 5 (HAdV-5) E1A protein has a well defined canonical nuclear localization signal (NLS) located at its C-terminus. We used a genetic assay in the yeast Saccharomyces cerevisiae to demonstrate that the canonical NLS is present and functional in the E1A proteins of each of the six HAdV species. This assay also detects a previously described non-canonical NLS within conserved region 3 and a novel active NLS within the N-terminal/conserved region 1 portion of HAdV-5 E1A. These activities were also present in the E1A proteins of each of the other five HAdV species. These results demonstrate that, despite substantial differences in primary sequence, HAdV E1A proteins are remarkably consistent in that they contain one canonical and two non-canonical NLSs. By utilizing independent mechanisms, these multiple NLSs ensure nuclear localization of E1A in the infected cell. - Highlights: • HAdV E1A uses multiple mechanisms for nuclear import. • We identified an additional non-canonical NLS in the N-terminal/CR1 portion of E1A. • The new NLS does not contact importin-alpha directly. • All NLSs are functionally conserved in the E1A proteins of all 6 HAdV species.
Authors:
; ; ; ;  [1] ;  [2] ;  [3] ;  [1] ;  [4]
  1. Department of Microbiology and Immunology, Western University, London Regional Cancer Program, London, ON, Canada N6A 4L6 (Canada)
  2. Department of Chemical and Environmental Engineering, Masdar Institute, Abu Dhabi (United Arab Emirates)
  3. College of Animal Science and Technologies, Northwest A and F University, Yangling, Shaanxi 712100 (China)
  4. (Canada)
Publication Date:
OSTI Identifier:
22435029
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 454-455; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADENOVIRUS; DNA; HUMAN POPULATIONS; MALTOSE; PROTEINS; RESIDUES; SACCHAROMYCES CEREVISIAE; SIGNALS; TRANSLOCATION