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Title: Analysis of crystallization data in the Protein Data Bank

In a large-scale study using data from the Protein Data Bank, some of the many reported findings regarding the crystallization of proteins were investigated. The Protein Data Bank (PDB) is the largest available repository of solved protein structures and contains a wealth of information on successful crystallization. Many centres have used their own experimental data to draw conclusions about proteins and the conditions in which they crystallize. Here, data from the PDB were used to reanalyse some of these results. The most successful crystallization reagents were identified, the link between solution pH and the isoelectric point of the protein was investigated and the possibility of predicting whether a protein will crystallize was explored.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [3]
  1. University of York, York YO10 5DD (United Kingdom)
  2. AstraZeneca, Darwin Building, Cambridge Science Park, Cambridge CB4 0WG (United Kingdom)
  3. (United Kingdom)
Publication Date:
OSTI Identifier:
22420143
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F, Structural Biology Communications; Journal Volume: 71; Journal Issue: Pt 10; Other Information: PMCID: PMC4601584; PMID: 26457511; PUBLISHER-ID: nj5235; PUBLISHER-ID: S2053230X15014892; OAI: oai:pubmedcentral.nih.gov:4601584; Copyright (c) Kirkwood et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; EXPERIMENTAL DATA; PH VALUE; PROTEIN STRUCTURE; STATISTICS