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Title: Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens

The crystallization of the novel virulence factors SghA and SghR is reported. Two proteins, SghA and SghR, which were recently identified and characterized as novel bacterial virulence factors regulating the infection of plant hosts by Agrobacterium, were cloned, overexpressed and purified with high yield. Both SghA and SghR form dimers in solution. The purified SghA and SghR were crystallized and the crystals diffracted to 1.9 and 2.1 Å resolution, respectively. Data were collected and processed, and the crystallographic parameters were within acceptable ranges. These results will help in the determination of their structures in order to uncover the molecular mechanism of how these two proteins together control the release of plant defence signals against agrobacteria during pathogen–host interaction.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [2] ;  [1] ;  [3]
  1. Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 (Singapore)
  2. Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673 (Singapore)
  3. (Singapore)
Publication Date:
OSTI Identifier:
22420140
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F, Structural Biology Communications; Journal Volume: 71; Journal Issue: Pt 9; Other Information: PMCID: PMC4555920; PMID: 26323299; PUBLISHER-ID: tt5072; PUBLISHER-ID: S2053230X15012881; OAI: oai:pubmedcentral.nih.gov:4555920; Copyright (c) Ye et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; INTERACTIONS; PURIFICATION; SIGNALS