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Title: Comparison of the biological effects of {sup 18}F at different intracellular levels

Abstract

Highlights: • We estimated the inductions of DNA DSB in cell treated with {sup 18}F-FDG. • We found that inductions of DNA DSB are dependent on accumulation of {sup 18}F in cell. • Accumulation of {sup 18}F in cell may be indispensable for risk estimation of PET. - Abstract: We herein examined the biological effects of cells treated with {sup 18}F labeled drugs for positron emission tomography (PET). The relationship between the intracellular distribution of {sup 18}F and levels of damaged DNA has yet to be clarified in detail. We used culture cells (Chinese Hamster Ovary cells) treated with two types of {sup 18}F labeled drugs, fluorodeoxyglucose (FDG) and fluorine ion (HF). FDG efficiently accumulated in cells, whereas HF did not. To examine the induction of DNA double strand breaks (DSB), we measured the number of foci for 53BP1 that formed at the site of DNA DSB. The results revealed that although radioactivity levels were the same, the induction of 53BP1 foci was stronger in cells treated with {sup 18}F-FDG than in those treated with {sup 18}F-HF. The clonogenic survival of cells was significantly lower with {sup 18}F-FDG than with {sup 18}F-HF. We concluded that the efficient accumulation of {supmore » 18}F in cells led to stronger biological effects due to more severe cellular lethality via the induction of DNA DSB.« less

Authors:
 [1]; ;  [1]; ;  [2]
  1. Advanced Molecular Imaging Center, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu City, Oita 879-5593 (Japan)
  2. Department of Radiology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu City, Oita 879-5593 (Japan)
Publication Date:
OSTI Identifier:
22416817
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 454; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL RADIATION EFFECTS; BUILDUP; CELL CULTURES; CHO CELLS; COMPARATIVE EVALUATIONS; DNA; FLUORINE 18; FLUORINE IONS; FLUORODEOXYGLUCOSE; HYDROFLUORIC ACID; INDUCTION; POSITRON COMPUTED TOMOGRAPHY; POSITRONS; RADIOACTIVITY; RADIOBIOLOGY; STRAND BREAKS

Citation Formats

Kashino, Genro, Hayashi, Kazutaka, Douhara, Kazumasa, Kobashigawa, Shinko, and Mori, Hiromu. Comparison of the biological effects of {sup 18}F at different intracellular levels. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.09.136.
Kashino, Genro, Hayashi, Kazutaka, Douhara, Kazumasa, Kobashigawa, Shinko, & Mori, Hiromu. Comparison of the biological effects of {sup 18}F at different intracellular levels. United States. https://doi.org/10.1016/J.BBRC.2014.09.136
Kashino, Genro, Hayashi, Kazutaka, Douhara, Kazumasa, Kobashigawa, Shinko, and Mori, Hiromu. 2014. "Comparison of the biological effects of {sup 18}F at different intracellular levels". United States. https://doi.org/10.1016/J.BBRC.2014.09.136.
@article{osti_22416817,
title = {Comparison of the biological effects of {sup 18}F at different intracellular levels},
author = {Kashino, Genro and Hayashi, Kazutaka and Douhara, Kazumasa and Kobashigawa, Shinko and Mori, Hiromu},
abstractNote = {Highlights: • We estimated the inductions of DNA DSB in cell treated with {sup 18}F-FDG. • We found that inductions of DNA DSB are dependent on accumulation of {sup 18}F in cell. • Accumulation of {sup 18}F in cell may be indispensable for risk estimation of PET. - Abstract: We herein examined the biological effects of cells treated with {sup 18}F labeled drugs for positron emission tomography (PET). The relationship between the intracellular distribution of {sup 18}F and levels of damaged DNA has yet to be clarified in detail. We used culture cells (Chinese Hamster Ovary cells) treated with two types of {sup 18}F labeled drugs, fluorodeoxyglucose (FDG) and fluorine ion (HF). FDG efficiently accumulated in cells, whereas HF did not. To examine the induction of DNA double strand breaks (DSB), we measured the number of foci for 53BP1 that formed at the site of DNA DSB. The results revealed that although radioactivity levels were the same, the induction of 53BP1 foci was stronger in cells treated with {sup 18}F-FDG than in those treated with {sup 18}F-HF. The clonogenic survival of cells was significantly lower with {sup 18}F-FDG than with {sup 18}F-HF. We concluded that the efficient accumulation of {sup 18}F in cells led to stronger biological effects due to more severe cellular lethality via the induction of DNA DSB.},
doi = {10.1016/J.BBRC.2014.09.136},
url = {https://www.osti.gov/biblio/22416817}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 454,
place = {United States},
year = {Fri Nov 07 00:00:00 EST 2014},
month = {Fri Nov 07 00:00:00 EST 2014}
}