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Title: miR-339-5p inhibits alcohol-induced brain inflammation through regulating NF-κB pathway

Graphical abstract: - Highlights: • Alcohol upregulates miR-339-5p expression. • miR-339-5p inhibits the NF-kB pathway. • miR-339-5p interacts with and blocks activity of IKK-beat and IKK-epsilon. • miR-339-5p modulates IL-1β, IL-6 and TNF-α. - Abstract: Alcohol-induced neuroinflammation is mediated by the innate immunesystem. Pro-inflammatory responses to alcohol are modulated by miRNAs. The miRNA miR-339-5p has previously been found to be upregulated in alcohol-induced neuroinflammation. However, little has been elucidated on the regulatory functions of this miRNA in alcohol-induced neuroinflammation. We investigated the function of miR-339-5p in alcohol exposed brain tissue and isolated microglial cells using ex vivo and in vitro techniques. Our results show that alcohol induces transcription of miR 339-5p, IL-6, IL-1β and TNF-α in mouse brain tissue and isolated microglial cells by activating NF-κB. Alcohol activation of NF-κB allows for nuclear translocation of the NF-κB subunit p65 and expression of pro-inflammatory mediators. miR-339-5p inhibited expression of these pro-inflammatory factors through the NF-κB pathway by abolishing IKK-β and IKK-ε activity.
Authors:
 [1] ;  [2] ;  [3] ;  [1]
  1. Department of Neurology, The First Affiliated School of Harbin Medical University, Harbin 150001 (China)
  2. Department of Neurology, Heilongjiang Provincial Hospital, Harbin 150036 (China)
  3. Department of Laboratory, Heilongjiang Provincial Hospital, Harbin 150036 (China)
Publication Date:
OSTI Identifier:
22416747
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 452; Journal Issue: 3; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALCOHOLS; ANIMAL TISSUES; BIOLOGICAL PATHWAYS; BRAIN; IN VITRO; INFLAMMATION; MICE; RNA; TRANSCRIPTION; TRANSLOCATION