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Title: Intracellular calcium levels can regulate Importin-dependent nuclear import

Abstract

Highlights: • High intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import. • The effect of Ca{sup 2+} on nuclear import does not relate to changes in the nuclear pore. • High intracellular calcium can result in mislocalisation of Impβ1, Ran and RCC1. - Abstract: We previously showed that increased intracellular calcium can modulate Importin (Imp)β1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impβ1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.

Authors:
;
Publication Date:
OSTI Identifier:
22416659
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 450; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CALCIUM IONS; CELL NUCLEI; CHROMATIN; HUMAN CHROMOSOMES; MEMBRANE TRANSPORT; PROTEINS

Citation Formats

Kaur, Gurpreet, Ly-Huynh, Jennifer D., and Jans, David A., E-mail: David.Jans@monash.edu. Intracellular calcium levels can regulate Importin-dependent nuclear import. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.06.047.
Kaur, Gurpreet, Ly-Huynh, Jennifer D., & Jans, David A., E-mail: David.Jans@monash.edu. Intracellular calcium levels can regulate Importin-dependent nuclear import. United States. https://doi.org/10.1016/J.BBRC.2014.06.047
Kaur, Gurpreet, Ly-Huynh, Jennifer D., and Jans, David A., E-mail: David.Jans@monash.edu. 2014. "Intracellular calcium levels can regulate Importin-dependent nuclear import". United States. https://doi.org/10.1016/J.BBRC.2014.06.047.
@article{osti_22416659,
title = {Intracellular calcium levels can regulate Importin-dependent nuclear import},
author = {Kaur, Gurpreet and Ly-Huynh, Jennifer D. and Jans, David A., E-mail: David.Jans@monash.edu},
abstractNote = {Highlights: • High intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import. • The effect of Ca{sup 2+} on nuclear import does not relate to changes in the nuclear pore. • High intracellular calcium can result in mislocalisation of Impβ1, Ran and RCC1. - Abstract: We previously showed that increased intracellular calcium can modulate Importin (Imp)β1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impα/β1- or Impβ1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impβ1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.},
doi = {10.1016/J.BBRC.2014.06.047},
url = {https://www.osti.gov/biblio/22416659}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 450,
place = {United States},
year = {Fri Jul 18 00:00:00 EDT 2014},
month = {Fri Jul 18 00:00:00 EDT 2014}
}