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Title: Cerebellar stem cells do not produce neurons and astrocytes in adult mouse

Abstract

Highlights: • No new neurons and astrocytes are generated in adult mouse cerebellum. • Very few mash1{sup +} or nestin{sup +} stem cells exist, and most of them are quiescent. • Cell proliferation rate is diversified among cerebellar regions and decreases over time. - Abstract: Although previous studies implied that cerebellar stem cells exist in some adult mammals, little is known about whether these stem cells can produce new neurons and astrocytes. In this study by bromodeoxyuridine (BrdU) intraperitoneal (i.p.) injection, we found that there are abundant BrdU{sup +} cells in adult mouse cerebellum, and their quantity and density decreases significantly over time. We also found cell proliferation rate is diversified in different cerebellar regions. Among these BrdU{sup +} cells, very few are mash1{sup +} or nestin{sup +} stem cells, and the vast majority of cerebellar stem cells are quiescent. Data obtained by in vivo retrovirus injection indicate that stem cells do not produce neurons and astrocytes in adult mouse cerebellum. Instead, some cells labeled by retrovirus are Iba1{sup +} microglia. These results indicate that very few stem cells exist in adult mouse cerebellum, and none of these stem cells contribute to neurogenesis and astrogenesis under physiological condition.

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22416632
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 450; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADULTS; BUDR; CELL NUCLEI; CELL PROLIFERATION; CEREBELLUM; FLUORESCENCE; IN VIVO; INJECTION; MICE; MOLECULES; NERVE CELLS; PROTEINS; STEM CELLS

Citation Formats

Su, Xin, Guan, Wuqiang, Yu, Yong-Chun, and Fu, Yinghui. Cerebellar stem cells do not produce neurons and astrocytes in adult mouse. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.05.131.
Su, Xin, Guan, Wuqiang, Yu, Yong-Chun, & Fu, Yinghui. Cerebellar stem cells do not produce neurons and astrocytes in adult mouse. United States. https://doi.org/10.1016/J.BBRC.2014.05.131
Su, Xin, Guan, Wuqiang, Yu, Yong-Chun, and Fu, Yinghui. 2014. "Cerebellar stem cells do not produce neurons and astrocytes in adult mouse". United States. https://doi.org/10.1016/J.BBRC.2014.05.131.
@article{osti_22416632,
title = {Cerebellar stem cells do not produce neurons and astrocytes in adult mouse},
author = {Su, Xin and Guan, Wuqiang and Yu, Yong-Chun and Fu, Yinghui},
abstractNote = {Highlights: • No new neurons and astrocytes are generated in adult mouse cerebellum. • Very few mash1{sup +} or nestin{sup +} stem cells exist, and most of them are quiescent. • Cell proliferation rate is diversified among cerebellar regions and decreases over time. - Abstract: Although previous studies implied that cerebellar stem cells exist in some adult mammals, little is known about whether these stem cells can produce new neurons and astrocytes. In this study by bromodeoxyuridine (BrdU) intraperitoneal (i.p.) injection, we found that there are abundant BrdU{sup +} cells in adult mouse cerebellum, and their quantity and density decreases significantly over time. We also found cell proliferation rate is diversified in different cerebellar regions. Among these BrdU{sup +} cells, very few are mash1{sup +} or nestin{sup +} stem cells, and the vast majority of cerebellar stem cells are quiescent. Data obtained by in vivo retrovirus injection indicate that stem cells do not produce neurons and astrocytes in adult mouse cerebellum. Instead, some cells labeled by retrovirus are Iba1{sup +} microglia. These results indicate that very few stem cells exist in adult mouse cerebellum, and none of these stem cells contribute to neurogenesis and astrogenesis under physiological condition.},
doi = {10.1016/J.BBRC.2014.05.131},
url = {https://www.osti.gov/biblio/22416632}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 450,
place = {United States},
year = {Fri Jul 18 00:00:00 EDT 2014},
month = {Fri Jul 18 00:00:00 EDT 2014}
}