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Title: Tetraspanin CD9 modulates human lymphoma cellular proliferation via histone deacetylase activity

Highlights: • CD9 is differentially expressed in human Burkitt’s lymphoma cells. • We found that CD9 expression promotes these cells proliferation. • CD9 expression also increases HDAC activity. • HDAC inhibition decreased both cell proliferation and importantly CD9 expression. • CD9 may dictate HDAC efficacy and play a role in HDAC regulation. - Abstract: Non-Hodgkin Lymphoma (NHL) is a type of hematological malignancy that affects two percent of the overall population in the United States. Tetraspanin CD9 is a cell surface protein that has been thoroughly demonstrated to be a molecular facilitator of cellular phenotype. CD9 expression varies in two human lymphoma cell lines, Raji and BJAB. In this report, we investigated the functional relationship between CD9 and cell proliferation regulated by histone deacetylase (HDAC) activity in these two cell lines. Introduction of CD9 expression in Raji cells resulted in significantly increased cell proliferation and HDAC activity compared to Mock transfected Raji cells. The increase in CD9–Raji cell proliferation was significantly inhibited by HDAC inhibitor (HDACi) treatment. Pretreatment of BJAB cells with HDAC inhibitors resulted in a significant decrease in endogenous CD9 mRNA and cell surface expression. BJAB cells also displayed decreased cell proliferation after HDACi treatment. These results suggestmore » a significant relationship between CD9 expression and cell proliferation in human lymphoma cells that may be modulated by HDAC activity.« less
Authors:
 [1] ;  [2] ;  [2] ;  [2] ; ;  [1] ;  [3] ; ;  [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [2]
  1. Vascular Biology Center of Excellence, The University of Tennessee Health Science Center, Memphis, TN 38163 (United States)
  2. (United States)
  3. Department of Biology, Bioinformatics Program, University of Memphis, Memphis, TN 38152 (United States)
Publication Date:
OSTI Identifier:
22416432
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 447; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; HEMATOLOGY; HUMAN POPULATIONS; INHIBITION; LYMPHOMAS; MESSENGER-RNA; PHENOTYPE; PROTEINS