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Title: DDX4 (DEAD box polypeptide 4) colocalizes with cancer stem cell marker CD133 in ovarian cancers

Highlights: • Germ cell marker DDX4 was significantly increased in ovarian cancer. • Ovarian cancer stem cell marker CD133 was significantly increased in ovarian cancer. • DDX4 and CD133 were mostly colocalized in various types of ovarian cancer tissues. • CD133 positive ovarian cancer cells also express DDX4 whereas CD133-negative cells did not possess DDX4. • Germ cell marker DDX4 has the potential of ovarian cancer stem cell marker. - Abstract: DDX4 (DEAD box polypeptide 4), characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an RNA helicase which is implicated in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX4 is known to be a germ cell-specific protein and is used as a sorting marker of germline stem cells for the production of oocytes. A recent report about DDX4 in ovarian cancer showed that DDX4 is overexpressed in epithelial ovarian cancer and disrupts a DNA damage-induced G2 checkpoint. We investigated the relationship between DDX4 and ovarian cancer stem cells by analyzing the expression patterns of DDX4 and the cancer stem cell marker CD133 in ovarian cancers via tissue microarray. Both DDX4 and CD133 were significantlymore » increased in ovarian cancer compared to benign tumors, and showed similar patterns of expression. In addition, DDX4 and CD133 were mostly colocalized in various types of ovarian cancer tissues. Furthermore, almost all CD133 positive ovarian cancer cells also express DDX4 whereas CD133-negative cells did not possess DDX4, suggesting a strong possibility that DDX4 plays an important role in cancer stem cells, and/or can be used as an ovarian cancer stem cell marker.« less
Authors:
 [1] ;  [2] ; ; ;  [3] ; ; ;  [1] ;  [2] ;  [4] ;  [5] ;  [6] ;  [7] ;  [3] ;  [2]
  1. Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Busan (Korea, Republic of)
  2. (Korea, Republic of)
  3. Department of Parasitology and Genetics, Kosin University College of Medicine, Busan (Korea, Republic of)
  4. Vincent Center for Reproductive Biology, Massachusetts General Hospital, Harvard Medical School, MA (United States)
  5. Department of Obstetrics and Gynecology, The Catholic University, Seoul (Korea, Republic of)
  6. Department of Internal Medicine, Kyung Hee University, Seoul (Korea, Republic of)
  7. Department of Obstetrics and Gynecology, Kosin University College of Medicine, Busan (Korea, Republic of)
Publication Date:
OSTI Identifier:
22416415
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 447; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; BIOLOGICAL MARKERS; COMPARATIVE EVALUATIONS; DNA DAMAGES; MITOCHONDRIA; NEOPLASMS; OOCYTES; OVARIES; POLYPEPTIDES; RNA; SPLICING; STEM CELLS