skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Downregulation of adenomatous polyposis coli by microRNA-663 promotes odontogenic differentiation through activation of Wnt/beta-catenin signaling

Abstract

Highlights: • miR-663 is significantly up-regulated during MDPC-23 odontoblastic cell differentiation. • miR-663 accelerates mineralization in MDPC-23 odontoblastic cells without cell proliferation. • miR-663 promotes odontoblastic cell differentiation by targeting APC and activating Wnt/β-catenin signaling in MDPC-23 cells. - Abstract: MicroRNAs (miRNAs) regulate cell differentiation by inhibiting mRNA translation or by inducing its degradation. However, the role of miRNAs in odontogenic differentiation is largely unknown. In this present study, we observed that the expression of miR-663 increased significantly during differentiation of MDPC-23 cells to odontoblasts. Furthermore, up-regulation of miR-663 expression promoted odontogenic differentiation and accelerated mineralization without proliferation in MDPC-23 cells. In addition, target gene prediction for miR-663 revealed that the mRNA of the adenomatous polyposis coli (APC) gene, which is associated with the Wnt/β-catenin signaling pathway, has a miR-663 binding site in its 3′-untranslated region (3′UTR). Furthermore, APC expressional was suppressed significantly by miR-663, and this down-regulation of APC expression triggered activation of Wnt/β-catenin signaling through accumulation of β-catenin in the nucleus. Taken together, these findings suggest that miR-663 promotes differentiation of MDPC-23 cells to odontoblasts by targeting APC-mediated activation of Wnt/β-catenin signaling. Therefore, miR-663 can be considered a critical regulator of odontoblast differentiation and can be utilized formore » developing miRNA-based therapeutic agents.« less

Authors:
; ; ; ; ; ; ; ; ; ; ;  [1];  [2];  [3]
  1. Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 501-759 (Korea, Republic of)
  2. Department of Biomedical Science, Chosun University, Gwangju 501-759 (Korea, Republic of)
  3. Department of Oral Histology-Developmental Biology, School of Dentistry and Dental Research Institute, BK 21, Seoul National University, Seoul 110-749 (Korea, Republic of)
Publication Date:
OSTI Identifier:
22416377
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 446; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL CELLS; BUILDUP; CELL DIFFERENTIATION; CELL NUCLEI; CELL PROLIFERATION; DRUGS; GENES; MESSENGER-RNA; MINERALIZATION

Citation Formats

Kim, Jae-Sung, Park, Min-Gyeong, Lee, Seul Ah, Park, Sun-Young, Kim, Heung-Joong, Yu, Sun-Kyoung, Kim, Chun Sung, Kim, Su-Gwan, Oh, Ji-Su, You, Jae-Seek, Kim, Jin-Soo, Seo, Yo-Seob, Chun, Hong Sung, Park, Joo-Cheol, and Kim, Do Kyung, E-mail: kdk@chosun.ac.kr. Downregulation of adenomatous polyposis coli by microRNA-663 promotes odontogenic differentiation through activation of Wnt/beta-catenin signaling. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.03.027.
Kim, Jae-Sung, Park, Min-Gyeong, Lee, Seul Ah, Park, Sun-Young, Kim, Heung-Joong, Yu, Sun-Kyoung, Kim, Chun Sung, Kim, Su-Gwan, Oh, Ji-Su, You, Jae-Seek, Kim, Jin-Soo, Seo, Yo-Seob, Chun, Hong Sung, Park, Joo-Cheol, & Kim, Do Kyung, E-mail: kdk@chosun.ac.kr. Downregulation of adenomatous polyposis coli by microRNA-663 promotes odontogenic differentiation through activation of Wnt/beta-catenin signaling. United States. https://doi.org/10.1016/J.BBRC.2014.03.027
Kim, Jae-Sung, Park, Min-Gyeong, Lee, Seul Ah, Park, Sun-Young, Kim, Heung-Joong, Yu, Sun-Kyoung, Kim, Chun Sung, Kim, Su-Gwan, Oh, Ji-Su, You, Jae-Seek, Kim, Jin-Soo, Seo, Yo-Seob, Chun, Hong Sung, Park, Joo-Cheol, and Kim, Do Kyung, E-mail: kdk@chosun.ac.kr. 2014. "Downregulation of adenomatous polyposis coli by microRNA-663 promotes odontogenic differentiation through activation of Wnt/beta-catenin signaling". United States. https://doi.org/10.1016/J.BBRC.2014.03.027.
@article{osti_22416377,
title = {Downregulation of adenomatous polyposis coli by microRNA-663 promotes odontogenic differentiation through activation of Wnt/beta-catenin signaling},
author = {Kim, Jae-Sung and Park, Min-Gyeong and Lee, Seul Ah and Park, Sun-Young and Kim, Heung-Joong and Yu, Sun-Kyoung and Kim, Chun Sung and Kim, Su-Gwan and Oh, Ji-Su and You, Jae-Seek and Kim, Jin-Soo and Seo, Yo-Seob and Chun, Hong Sung and Park, Joo-Cheol and Kim, Do Kyung, E-mail: kdk@chosun.ac.kr},
abstractNote = {Highlights: • miR-663 is significantly up-regulated during MDPC-23 odontoblastic cell differentiation. • miR-663 accelerates mineralization in MDPC-23 odontoblastic cells without cell proliferation. • miR-663 promotes odontoblastic cell differentiation by targeting APC and activating Wnt/β-catenin signaling in MDPC-23 cells. - Abstract: MicroRNAs (miRNAs) regulate cell differentiation by inhibiting mRNA translation or by inducing its degradation. However, the role of miRNAs in odontogenic differentiation is largely unknown. In this present study, we observed that the expression of miR-663 increased significantly during differentiation of MDPC-23 cells to odontoblasts. Furthermore, up-regulation of miR-663 expression promoted odontogenic differentiation and accelerated mineralization without proliferation in MDPC-23 cells. In addition, target gene prediction for miR-663 revealed that the mRNA of the adenomatous polyposis coli (APC) gene, which is associated with the Wnt/β-catenin signaling pathway, has a miR-663 binding site in its 3′-untranslated region (3′UTR). Furthermore, APC expressional was suppressed significantly by miR-663, and this down-regulation of APC expression triggered activation of Wnt/β-catenin signaling through accumulation of β-catenin in the nucleus. Taken together, these findings suggest that miR-663 promotes differentiation of MDPC-23 cells to odontoblasts by targeting APC-mediated activation of Wnt/β-catenin signaling. Therefore, miR-663 can be considered a critical regulator of odontoblast differentiation and can be utilized for developing miRNA-based therapeutic agents.},
doi = {10.1016/J.BBRC.2014.03.027},
url = {https://www.osti.gov/biblio/22416377}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 446,
place = {United States},
year = {Fri Apr 18 00:00:00 EDT 2014},
month = {Fri Apr 18 00:00:00 EDT 2014}
}