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Title: Mining secreted proteins that function in pepper fruit development and ripening using a yeast secretion trap (YST)

Highlights: • Yeast secretion trap (YST) is a valuable tool for mining secretome. • A total of 80 secreted proteins are newly identified via YST in pepper fruits. • The secreted proteins are differentially regulated during pepper development and ripening. • Transient GFP-fusion assay and in planta secretion trap can effectively validate the secretion of proteins. - Abstract: Plant cells secrete diverse sets of constitutively- and conditionally-expressed proteins under various environmental and developmental states. Secreted protein populations, or secretomes have multiple functions, including defense responses, signaling, metabolic processes, and developmental regulation. To identify genes encoding secreted proteins that function in fruit development and ripening, a yeast secretion trap (YST) screen was employed using pepper (Capsicum annuum) fruit cDNAs. The YST screen revealed 80 pepper fruit-related genes (CaPFRs) encoding secreted proteins including cell wall proteins, several of which have not been previously described. Transient GFP-fusion assay and an in planta secretion trap were used to validate the secretion of proteins encoded by selected YST clones. In addition, RNA gel blot analyses provided further insights into their expression and regulation during fruit development and ripening. Integrating our data, we conclude that the YST provides a valuable functional genomics tool for the identificationmore » of substantial numbers of novel secreted plant proteins that are associated with biological processes, including fruit development and ripening.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [1]
  1. Department of Plant Science, College of Agriculture and Life Sciences, Seoul National University, Seoul (Korea, Republic of)
  2. (Korea, Republic of)
  3. Biotechnology Institute, Nongwoo Bio Co, Ltd, Yeoju (Korea, Republic of)
  4. (United States)
  5. Department of Plant Biology, Cornell University, Ithaca, NY (United States)
  6. Department of Horticulture and Breeding, Andong National University, Andong (Korea, Republic of)
Publication Date:
OSTI Identifier:
22416375
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 446; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CAPSICUM; CELL WALL; FRUITS; GELS; GENES; PEPPERS; PROTEINS; RNA; SECRETION; SOCIO-ECONOMIC FACTORS; TRANSIENTS; TRAPS; YEASTS