skip to main content

Title: Detection of PIWI and piRNAs in the mitochondria of mammalian cancer cells

Highlights: • piRNA sequences were mapped to human mitochondrial (mt) genome. • We inspected small RNA-Seq datasets from somatic cell mt subcellular fractions. • Piwi and piRNA transcripts are present in mammalian somatic cancer cell mt fractions. - Abstract: Piwi-interacting RNAs (piRNAs) are 26–31 nt small noncoding RNAs that are processed from their longer precursor transcripts by Piwi proteins. Localization of Piwi and piRNA has been reported mostly in nucleus and cytoplasm of higher eukaryotes germ-line cells, where it is believed that known piRNA sequences are located in repeat regions of nuclear genome in germ-line cells. However, localization of PIWI and piRNA in mammalian somatic cell mitochondria yet remains largely unknown. We identified 29 piRNA sequence alignments from various regions of the human mitochondrial genome. Twelve out 29 piRNA sequences matched stem-loop fragment sequences of seven distinct tRNAs. We observed their actual expression in mitochondria subcellular fractions by inspecting mitochondrial-specific small RNA-Seq datasets. Of interest, the majority of the 29 piRNAs overlapped with multiple longer transcripts (expressed sequence tags) that are unique to the human mitochondrial genome. The presence of mature piRNAs in mitochondria was detected by qRT-PCR of mitochondrial subcellular RNAs. Further validation showed detection of Piwi by colocalizationmore » using anti-Piwil1 and mitochondria organelle-specific protein antibodies.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [4] ;  [5] ;  [6] ;  [2] ;  [1]
  1. Cancer Genomics Branch, National Cancer Center, Goyang 410-769 (Korea, Republic of)
  2. Department of Biochemistry, College of Medicine, Catholic University of Korea, Seoul 137-701 (Korea, Republic of)
  3. Department of Computer Science, Hanyang University, Seoul 133-791 (Korea, Republic of)
  4. New Experimental Therapeutics Branch, National Cancer Center, Goyang 410-769 (Korea, Republic of)
  5. Molecular Epidemiology Branch, National Cancer Center, Goyang 410-769 (Korea, Republic of)
  6. Medical Sciences Program, Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Bloomington, IN 47405 (United States)
Publication Date:
OSTI Identifier:
22416339
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 446; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; CELL NUCLEI; CYTOPLASM; HUMAN POPULATIONS; MITOCHONDRIA; NEOPLASMS; POLYMERASE CHAIN REACTION; PRECURSOR; PROTEINS; RNA; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS