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Title: MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1

Abstract

Highlights: • We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line. • We are the first to show the function of miR-490-3p in A549 lung cancer cells. • We demonstrate CCND1 may be one of the targets of miR-490-3p. - Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation.

Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
22416253
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 444; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; FUNCTIONS; GAIN; LUCIFERASE; LUNGS; MESSENGER-RNA; NEOPLASMS; PHASE TRANSFORMATIONS

Citation Formats

Gu, Haihua, Yang, Tao, Fu, Shaozi, Chen, Xiaofan, Guo, Lei, and Ni, Yiming. MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.01.020.
Gu, Haihua, Yang, Tao, Fu, Shaozi, Chen, Xiaofan, Guo, Lei, & Ni, Yiming. MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1. United States. https://doi.org/10.1016/J.BBRC.2014.01.020
Gu, Haihua, Yang, Tao, Fu, Shaozi, Chen, Xiaofan, Guo, Lei, and Ni, Yiming. 2014. "MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1". United States. https://doi.org/10.1016/J.BBRC.2014.01.020.
@article{osti_22416253,
title = {MicroRNA-490-3p inhibits proliferation of A549 lung cancer cells by targeting CCND1},
author = {Gu, Haihua and Yang, Tao and Fu, Shaozi and Chen, Xiaofan and Guo, Lei and Ni, Yiming},
abstractNote = {Highlights: • We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line. • We are the first to show the function of miR-490-3p in A549 lung cancer cells. • We demonstrate CCND1 may be one of the targets of miR-490-3p. - Abstract: MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation.},
doi = {10.1016/J.BBRC.2014.01.020},
url = {https://www.osti.gov/biblio/22416253}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 444,
place = {United States},
year = {Fri Jan 31 00:00:00 EST 2014},
month = {Fri Jan 31 00:00:00 EST 2014}
}