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Title: Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas

Highlights: • Most of the adenocarcinomas and bronchioloalveolar carcinomas were LIPH-positive. • LIPH is necessary for the proliferation of lung cancer cells in vitro. • A high level of LIPH in serum is correlated with better survival in early phase lung-cancer patients after surgery. - Abstract: Lung cancer is one of the most frequent causes of cancer-related death worldwide. However, molecular markers for lung cancer have not been well established. To identify novel genes related to lung cancer development, we surveyed publicly available DNA microarray data on lung cancer tissues. We identified lipase member H (LIPH, also known as mPA-PLA1) as one of the significantly upregulated genes in lung adenocarcinoma. LIPH was expressed in several adenocarcinoma cell lines when they were analyzed by quantitative real-time polymerase chain reaction (qPCR), western blotting, and sandwich enzyme-linked immunosorbent assay (ELISA). Immunohistochemical analysis detected LIPH expression in most of the adenocarcinomas and bronchioloalveolar carcinomas tissue sections obtained from lung cancer patients. LIPH expression was also observed less frequently in the squamous lung cancer tissue samples. Furthermore, LIPH protein was upregulated in the serum of early- and late-phase lung cancer patients when they were analyzed by ELISA. Interestingly, high serum level of LIPH was correlatedmore » with better survival in early phase lung cancer patients after surgery. Thus, LIPH may be a novel molecular biomarker for lung cancer, especially for adenocarcinoma and bronchioloalveolar carcinoma.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [5] ;  [4] ;  [6] ;  [5] ;  [7] ;  [8] ;  [6] ;  [1] ;  [1] ;  [2] ;  [9] ;  [5] ;  [4]
  1. Graduate School of Arts and Sciences, The University of Tokyo, Tokyo (Japan)
  2. (AIST), Tsukuba (Japan)
  3. Department of Surgery, Asahi General Hospital, Chiba (Japan)
  4. (Japan)
  5. Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba (Japan)
  6. Department of Pathology, Graduate School of Medicine, The University of Tokyo, Hongo, Tokyo (Japan)
  7. Department of Internal Medicine, JA Kochi Hospital, Kochi (Japan)
  8. Department of Thoracic Surgery, The University of Tokyo, Graduate School of Medicine, Tokyo (Japan)
  9. (TARA), The University of Tsukuba, Tsukuba, Ibaraki (Japan)
Publication Date:
OSTI Identifier:
22416240
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 443; Journal Issue: 4; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; BIOLOGICAL MARKERS; CARCINOMAS; CELL PROLIFERATION; DNA; ENZYME IMMUNOASSAY; GENES; HUMAN POPULATIONS; IN VITRO; LIPASES; LUNGS; PATIENTS; PLANT TISSUES; POLYMERASE CHAIN REACTION; SURGERY