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Title: Pf1 bacteriophage hydration by magic angle spinning solid-state NMR

High resolution two- and three-dimensional heteronuclear correlation spectroscopy ({sup 1}H–{sup 13}C, {sup 1}H–{sup 15}N, and {sup 1}H–{sup 13}C–{sup 13}C HETCOR) has provided a detailed characterization of the internal and external hydration water of the Pf1 virion. This long and slender virion (2000 nm × 7 nm) contains highly stretched DNA within a capsid of small protein subunits, each only 46 amino acid residues. HETCOR cross-peaks have been unambiguously assigned to 25 amino acids, including most external residues 1–21 as well as residues 39–40 and 43–46 deep inside the virion. In addition, the deoxyribose rings of the DNA near the virion axis are in contact with water. The sets of cross-peaks to the DNA and to all 25 amino acid residues were from the same hydration water {sup 1}H resonance; some of the assigned residues do not have exchangeable side-chain protons. A mapping of the contacts onto structural models indicates the presence of water “tunnels” through a highly hydrophobic region of the capsid. The present results significantly extend and modify results from a lower resolution study, and yield a comprehensive hydration surface map of Pf1. In addition, the internal water could be distinguished from external hydration water by means of paramagneticmore » relaxation enhancement. The internal water population may serve as a conveniently localized magnetization reservoir for structural studies.« less
Authors:
; ;  [1] ;  [2]
  1. Department of Chemistry, Columbia University, 3000 Broadway, New York, New York 10027 (United States)
  2. Public Health Research Institute, Rutgers University, 225 Warren St., Newark, New Jersey 07103 (United States)
Publication Date:
OSTI Identifier:
22413318
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Chemical Physics; Journal Volume: 141; Journal Issue: 22; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; AMINO ACIDS; BACTERIOPHAGES; CARBON 13; DEOXYRIBOSE; DNA; HYDRATION; MAGNETIZATION; NUCLEAR MAGNETIC RESONANCE; PARAMAGNETISM; PEAKS; PROTONS; RESIDUES; SOLIDS; SPECTROSCOPY; SURFACES; THREE-DIMENSIONAL LATTICES; WATER