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Title: Solid-immersion fluorescence microscopy with increased emission and super resolution

We investigate solid-immersion fluorescence microscopy suitable for super-resolution nanotechnology and biological imaging, and have observed limit of resolution as small as 15 nm with microspheres, mitochondria, and chromatin fibers. We have further observed that fluorescence efficiency increases with excitation power density, implicating appreciable stimulated emission and increased resolution. We discuss potential advantages of the solid-immersion microscopy, including combined use with previously established super-resolution techniques for reaching deeper beyond the conventional diffraction limit.
Authors:
;  [1] ; ;  [2] ;  [3] ;  [4]
  1. Lincoln Laboratory, Massachusetts Institute of Technology, Lexington, Massachusetts 02420 (United States)
  2. Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)
  3. Whitehead Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)
  4. Department of Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107 (United States)
Publication Date:
OSTI Identifier:
22399224
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Applied Physics; Journal Volume: 117; Journal Issue: 1; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; CHROMATIN; DIFFRACTION; EFFICIENCY; EXCITATION; FIBERS; FLUORESCENCE; MICROSCOPY; MICROSPHERES; MITOCHONDRIA; NANOTECHNOLOGY; POWER DENSITY; RESOLUTION; SOLIDS; STIMULATED EMISSION