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Title: Multi-images deconvolution improves signal-to-noise ratio on gated stimulated emission depletion microscopy

Time-gated detection, namely, only collecting the fluorescence photons after a time-delay from the excitation events, reduces complexity, cost, and illumination intensity of a stimulated emission depletion (STED) microscope. In the gated continuous-wave- (CW-) STED implementation, the spatial resolution improves with increased time-delay, but the signal-to-noise ratio (SNR) reduces. Thus, in sub-optimal conditions, such as a low photon-budget regime, the SNR reduction can cancel-out the expected gain in resolution. Here, we propose a method which does not discard photons, but instead collects all the photons in different time-gates and recombines them through a multi-image deconvolution. Our results, obtained on simulated and experimental data, show that the SNR of the restored image improves relative to the gated image, thereby improving the effective resolution.
Authors:
 [1] ;  [2] ;  [1] ;  [2] ;  [1]
  1. Nanobiophotonics, Nanophysics, Istituto Italiano di Tecnologia, Via Morego 30, Genoa, 16163 (Italy)
  2. (Italy)
Publication Date:
OSTI Identifier:
22395785
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 23; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; EXCITATION; FLUORESCENCE; GAIN; ILLUMINANCE; IMAGES; IMPLEMENTATION; MICROSCOPY; PHOTONS; SIGNAL-TO-NOISE RATIO; SPATIAL RESOLUTION; STIMULATED EMISSION; TIME DELAY