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Title: Plasmon resonance enhanced mechanical detection of ligand binding

Small molecule binding to the active site of enzymes typically modifies the mechanical stiffness of the enzyme. We exploit this effect, in a setup which combines nano-mechanics and surface plasmon resonance (SPR) enhanced optics, for the label free detection of ligand binding to an enzyme. The large dynamic range of the signal allows to easily obtain binding curves for small ligands, in contrast to traditional SPR methods which rely on small changes in index of refraction. Enzyme mechanics, assessed by nano-rheology, thus emerges as an alternative to electronic and spin resonances, assessed by traditional spectroscopies, for detecting ligand binding.
Authors:
;  [1]
  1. Department of Physics and Astronomy, University of California Los Angeles, Los Angeles, California 90095 (United States)
Publication Date:
OSTI Identifier:
22395679
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 106; Journal Issue: 1; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; DIAGRAMS; ENZYMES; FLEXIBILITY; LIGANDS; MOLECULES; NANOSTRUCTURES; OPTICS; PLASMONS; REFRACTIVE INDEX; RESONANCE; RHEOLOGY; SPECTROSCOPY; SPIN; SURFACES