skip to main content

Title: Hypericin-mediated selective photomodification of connective tissues

Controllable modification of biological molecules and supramolecular components of connective tissue are important for biophysical and biomedical applications. Through the use of second harmonic generation imaging, two-photon fluorescence microscopy, and spectrofluorimetry, we found that hypericin, a natural pigment, induces photosensitized destruction of collagen fibers but does not affect elastic fibers and lipids in chicken tendon, skin, and blood vessels. We demonstrated the dynamics and efficiency of collagen photomodification and investigated mechanisms of this processes. Our results suggest that hypericin–mediated photoprocesses in biological tissues may be useful in biomedical applications that require selective modification of connective tissues.
Authors:
; ;  [1] ;  [2] ;  [3] ;  [1] ;  [4]
  1. Department of Physics, National Taiwan University, Taipei 106, Taiwan (China)
  2. Multimedia and Programming, European Regional Education Academy, Yerevan 0037 (Armenia)
  3. Neuroscience Center, University of North Carolina at Chapel Hill, North Carolina 27514 (United States)
  4. (China)
Publication Date:
OSTI Identifier:
22395626
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 26; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; 60 APPLIED LIFE SCIENCES; BLOOD VESSELS; COLLAGEN; EFFICIENCY; FIBERS; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; HARMONIC GENERATION; LIPIDS; MICROSCOPY; MODIFICATIONS; MOLECULES; PHOTONS; PIGMENTS; SKIN; TENDONS